NMR solution structure of HP0827 (O25501-HELPY) from helicobacter pylori: Model of the possible RNA-binding site

Sun Bok Jang, Chao Ma, Ji Yoon Lee, Ji Hun Kim, Sung Jean Park, Ae Ran Kwon, Bong Jin Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The HP0827 protein is an 82-residue protein identified as a putative ss-DNA-binding protein 12RNP2 Precursor from Helicobacter pylori. Here, we have determined 3D structure of HP0827 using Nuclear Magnetic Resonance. It has a ferredoxin-like fold, β1α1β2β3α2β4 (α; α-helix and β; β-sheet) and ribonucleoprotein (RNP) motifs which are thought to be important in RNA binding. By using structural homologues search and analyzing electrostatic potential of surface, we could compared HP0827 with other RNA-binding proteins (sex-lethal, T-cell restricted intracellular antigen-1, U1A) to predict RNA-binding sites of HP0827. We could predict that β sheets of HP0827, especially β1 and β3, are primary region for RNA binding. Consequently, similar to other RNA-binding proteins, RNP motifs (Y5, F45, F47), positively charged and hydrophobic regions (K32, R37, K40, K41, K43, R70, R73) are proposed as a putative RNA-binding sites. In addition, differences in amino acids composition of RNP motifs, N, C-terminal residues, loop-region fold and the orientation of α1-helix with other RNA recognition motif proteins could give specific biological functions to HP0827. Finally, the study on natural RNA target is also important to completely understand the biological function of HP0827.

Original languageEnglish
Pages (from-to)667-674
Number of pages8
JournalJournal of Biochemistry
Issue number5
StatePublished - Nov 2009

Bibliographical note

Funding Information:
Ministry of Education, Science and Technology (MEST); New Drug Target Discovery; Grant number, 370C-20070095, Innovative Drug Research Center for Metabolic and Inflammatory Disease; and 2008 BK21 Project for Medicine, Dentistry and Pharmacy.


  • HP0827
  • Helicobacter pylori
  • RNP motif
  • RRM
  • Single-stranded DNA-binding proteins


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