NKG2D ligation relieves 2B4-mediated NK-cell self-tolerance in mice

Jung Eun Lee, Seon Ah Lim, Tae Jin Kim, Kwanghee Kim, Joanne Ng, Yong Ho Kim, In Jung Jang, Seog Bae Oh, June Chul Lee, Cassian Yee, Vinay Kumar, Kyung Mi Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Along with MHC class I (MHCI), 2B4 provides nonredundant NK-cell inhibition in mice. The immunoregulatory role of 2B4 has been increasingly appreciated in models of tumor and viral infection, however, the interactions among 2B4, MHCI, and other activating NK-cell receptors remain uncertain. Here, we dissect the influence of two distinct inhibitory pathways in modulating NK-cell-mediated control of tumors expressing strong activating ligands, including RAE-1γ. In vitro cytotoxicity and in vivo peritoneal clearance assays using MHCI+CD48+ (RMA-neo), MHCI+CD48+RAE-1γ (RMA-RAE-1γ), MHCI-CD48+ (RMA-S-neo), and MHCI-CD48+RAE-1γ (RMA-S-RAE-1γ) tumor lines demonstrated that NKG2D activation supersedes the inhibitory effect of both 2B4- and MHCI-mediated immune-tolerance systems. Furthermore, 2B4KO mice subcutaneously challenged with RMA-neo and RMA-S-neo exhibited reduced tumor growth and significantly prolonged survival compared with WT mice, implying that 2B4 is constitutively engaged in the NK-cell tolerance mechanism in vivo. Nevertheless, the inhibitory effect of 2B4 is significantly attenuated when NK cells encountered highly stressed tumor cells expressing RAE-1γ, resulting in an immune response shift toward NK-cell activation and tumor regression. Therefore, our data highlight the importance of the 2B4-mediated inhibitory system as an alternate self-tolerance mechanism, whose role can be modulated by the strength of activating receptor signaling within the tumor microenvironment.

Original languageEnglish
Pages (from-to)1802-1813
Number of pages12
JournalEuropean Journal of Immunology
Issue number6
StatePublished - Jun 2014


  • 2B4
  • NKG2D
  • Natural killer cells
  • Self-tolerance


Dive into the research topics of 'NKG2D ligation relieves 2B4-mediated NK-cell self-tolerance in mice'. Together they form a unique fingerprint.

Cite this