Nitrifying-genes dynamics in the enriched bacterial consortium inoculated with humic soil

Yoon Joo Seo, Yun Yeong Lee, Hyung Joo Choi, Kyung Suk Cho

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


In this study, the effects of ammonium concentration (117.5-1155.0 mg-N·1-1), nitrite concentration (0-50.0 mg-N·1-1), and temperature (15-35°C) on nitrification performance and its functional genes (amoA-arc, amoA-bac, hao) in an enriched consortium inoculated with humic acid were determined. Notably, the maximum nitrification rate value was observed at 315 mg-N·1-1 of ammonium, but the highest functional gene copy numbers were obtained at 630 mg-N·1-1 of ammonium. No inhibition of the nitrification rate and functional gene copy numbers was observed via the added nitrites. The optimum temperature for maximum nitrification performance was observed to be 30°C. The amoA-bac copy numbers were also greater than those of amoA-arc under all test conditions. Notably, amoA-arc copy numbers and nitrification efficiency showed a positive relationship in network analysis. These results indicate that ammonium-oxidizing archaea and bacteria play important roles in the nitrification process.

Original languageEnglish
Pages (from-to)296-302
Number of pages7
JournalKorean Journal of Applied Microbiology and Biotechnology
Issue number2
StatePublished - Jun 2019

Bibliographical note

Publisher Copyright:
© 2019, The Korean Society for Microbiology and Biotechnology.


  • Ammonium
  • Ammonium-oxidizing archaea
  • Ammonium-oxidizing bacteria
  • Functional gene
  • Nitrification
  • Nitrite


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