New platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

Jae Hyung Lee; Dong Heon Nam; Sahng Ha Lee; Jong Hyun Park; Si Jae Park; Seung Hwan Lee; Chan Beum Park; Ki Jun Jeong

doi:10.1021/bc500404j

New platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

Jae Hyung Lee, Dong Heon Nam, Sahng Ha Lee, Jong Hyun Park, Si Jae Park, Seung Hwan Lee, Chan Beum Park, Ki Jun Jeong

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450-P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450-P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved. (Figure Presented).

Original language	English
Pages (from-to)	2101-2104
Number of pages	4
Journal	Bioconjugate Chemistry
Volume	25
Issue number	12
DOIs	https://doi.org/10.1021/bc500404j
State	Published - 17 Dec 2014

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© 2014 American Chemical Society.

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@article{104016ba71d04889a61eae8d22ee1054,

title = "New platform for cytochrome P450 reaction combining in situ immobilization on biopolymer",

abstract = "We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450-P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450-P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved. (Figure Presented).",

author = "Lee, {Jae Hyung} and Nam, {Dong Heon} and Lee, {Sahng Ha} and Park, {Jong Hyun} and Park, {Si Jae} and Lee, {Seung Hwan} and Park, {Chan Beum} and Jeong, {Ki Jun}",

note = "Publisher Copyright: {\textcopyright} 2014 American Chemical Society.",

year = "2014",

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doi = "10.1021/bc500404j",

language = "English",

volume = "25",

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T1 - New platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

AU - Lee, Jae Hyung

AU - Nam, Dong Heon

AU - Lee, Sahng Ha

AU - Park, Jong Hyun

AU - Park, Si Jae

AU - Lee, Seung Hwan

AU - Park, Chan Beum

AU - Jeong, Ki Jun

PY - 2014/12/17

Y1 - 2014/12/17

N2 - We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450-P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450-P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved. (Figure Presented).

AB - We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450-P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450-P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved. (Figure Presented).

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VL - 25

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EP - 2104

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 12

ER -

New platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

Abstract

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