New platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

Jae Hyung Lee, Dong Heon Nam, Sahng Ha Lee, Jong Hyun Park, Si Jae Park, Seung Hwan Lee, Chan Beum Park, Ki Jun Jeong

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450-P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450-P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved. (Figure Presented).

Original languageEnglish
Pages (from-to)2101-2104
Number of pages4
JournalBioconjugate Chemistry
Volume25
Issue number12
DOIs
StatePublished - 17 Dec 2014

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