A novel O-butyryl chitosan (OBCS)-grafted polypyrrole (PPy) film was described. The immobilization was accomplished by photocrosslinking the OBCS onto PPy films under ultraviolet light irradiation. The surfaces of OBCS-grafted PPy film were characterized by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and electron spectroscopy for chemical analysis (ESCA). The blood compatibility of the OBCS-grafted PPy film was evaluated by platelet-rich plasma (PRP) contacting experiments and protein adsorption experiments in vitro. These results have demonstrated that the surface with immobilized OBCS shows much less platelet adhesive and fibrinogen adsorption compared to the control surface. The bulk conductivity values of PPy films were measured by a modified four-probe method. The composite films have both good blood compatibility and high electrical conductivity that make them suitable for using as potential biomaterials, such as electrically conducting blood vessel and functionally haemocompatible substrate of biosensor used directly in whole blood.
Bibliographical noteFunding Information:
This work was supported by the SRC/ERC program of MOST/KOSEF (R11-2005-008-000-0 to J.K.), Seoul R&BD program (10816 to J.K.), the Brain Korea 21 project (to Q. Wu), the National High Technology Research and Development Program of China (Grant No. 2006AA03Z445), and Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20050319010).
- Blood compatibility
- Electrical conductivity
- Fibrinogen adsorption
- Platelet adhesion
- Polypyrrole films