Neutrophil Recruitment in Arterial Thrombus and Characteristics of Stroke Patients with Neutrophil-Rich Thrombus

Myoung Jin Cha, Jimin Ha, Hyungwoo Lee, Il Kwon, Sungeun Kim, Young Dae Kim, Hyo Suk Nam, Hye Sun Lee, Tae Jin Song, Hyun Jung Choi, Ji Hoe Heo

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Purpose: Neutrophils contribute to thrombosis. However, there is limited information on the temporal course of neutrophil recruitment in thrombosis, the contribution of neutrophils to thrombus growth, and the characteristics of stroke patients with neu-trophil-rich thrombi. Materials and Methods: After inducing carotid artery thrombosis in Institute of Cancer Research mice using ferric chloride, aged thrombi were produced by ligating the distal portion of the carotid artery in mice for 0.5, 1, 2, 3, 6, or 24 h. For thrombus analysis in stroke patients, we used registry data and thrombi that were obtained during intra-arterial thrombectomy. Immunohisto-chemistry was performed to determine thrombus composition. Results: In the thrombi of 70 mice, Ly6G positive cell counts (neutrophils) and histone H3-positive cell counts increased in a time-dependent manner (both p<0.001). Ly6G-positive cell count was strongly correlated with histone H3-positive cell counts (r=0.910, p<0.001), but not with thrombus size (p=0.320). In 75 stroke patients, atrial fibrillation and cardioembolism were more fre-quent in the higher neutrophil group (32/37, 86.5%) than in the lower neutrophil group (19/38, 50%) (p=0.002). The median erythrocyte fraction was higher [52.0 (interquartile range 39.9−57.8)] in the higher neutrophil group than in the lower neutrophil group [40.3 (interquartile range 23.5−53.2)]. The fraction of neutrophils was positively correlated with that of erythrocytes (R=0.35, p=0.002). Conclusion: Neutrophils were recruited and increased in arterial thrombosis in a time-dependent manner; however, they were not associated with the growth of formed thrombi. Neutrophil fractions in the thrombi of stroke patients appeared to be associated with atrial fibrillation and erythrocyte fraction.

Original languageEnglish
Pages (from-to)1016-1026
Number of pages11
JournalYonsei Medical Journal
Volume63
Issue number11
DOIs
StatePublished - Nov 2022

Bibliographical note

Funding Information:
This study was supported by the Basic Science Research Pro-

Publisher Copyright:
© Yonsei University College of Medicine 2022.

Keywords

  • Neutrophil
  • intra-arterial thrombectomy
  • neutrophil extracellular traps
  • stroke
  • thrombosis

Fingerprint

Dive into the research topics of 'Neutrophil Recruitment in Arterial Thrombus and Characteristics of Stroke Patients with Neutrophil-Rich Thrombus'. Together they form a unique fingerprint.

Cite this