Neuroprotective effect of Cu,Zn-superoxide dismutase fused to a TCTP-derived protein transduction domain

Jisun Lee, Sabin Kim, Dong Hae Shin, Hwa Jung Kim, Kyunglim Lee

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Previously, we have reported that a 10-amino acid peptide (MIIYRDLISH) derived from the NH2-terminus of the human translationally controlled tumor protein (TCTP) functions as a protein transduction domain (PTD). In this study, we evaluated the transduction ability of SOD fused to TCTP-PTD (TCTP-SOD) into various cell lines. We also evaluated its ability to protect cells against paraquat-induced cell damage, in vitro and its neuroprotective effect in vivo against kainic acid-induced neuronal damage in an animal model. TCTP-SOD was transduced into various cell lines in a dose- and time-dependent manner without cytotoxic effect. Furthermore, TCTP-SOD showed cytoprotective activity in SH-SY5Y cells, and intraperitoneally, injected TCTP-SOD was delivered into the mouse brain and protected the cells in the hippocampal region against the damage induced by kainic acid. We propose TCTP-SOD as a potential candidate drug for treatment of brain diseases.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalEuropean Journal of Pharmacology
Volume666
Issue number1-3
DOIs
StatePublished - Sep 2011

Keywords

  • Kainic acid
  • Paraquat
  • Protein transduction domain
  • Superoxide dismutase
  • Translationally controlled tumor protein

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