TY - JOUR
T1 - Neuroprotective effect of Cu,Zn-superoxide dismutase fused to a TCTP-derived protein transduction domain
AU - Lee, Jisun
AU - Kim, Sabin
AU - Shin, Dong Hae
AU - Kim, Hwa Jung
AU - Lee, Kyunglim
N1 - Funding Information:
This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs (A090030), NRF of Korea Grant funded by the Korean Government (2009–0064401), Mid-career Research Program through NRF grant funded by the MEST (R01-2007-000-20263-0) , Seoul R&BD Program (ST090801), and the NCRC program of MOST/KOSEF (R15-2006-020).
PY - 2011/9
Y1 - 2011/9
N2 - Previously, we have reported that a 10-amino acid peptide (MIIYRDLISH) derived from the NH2-terminus of the human translationally controlled tumor protein (TCTP) functions as a protein transduction domain (PTD). In this study, we evaluated the transduction ability of SOD fused to TCTP-PTD (TCTP-SOD) into various cell lines. We also evaluated its ability to protect cells against paraquat-induced cell damage, in vitro and its neuroprotective effect in vivo against kainic acid-induced neuronal damage in an animal model. TCTP-SOD was transduced into various cell lines in a dose- and time-dependent manner without cytotoxic effect. Furthermore, TCTP-SOD showed cytoprotective activity in SH-SY5Y cells, and intraperitoneally, injected TCTP-SOD was delivered into the mouse brain and protected the cells in the hippocampal region against the damage induced by kainic acid. We propose TCTP-SOD as a potential candidate drug for treatment of brain diseases.
AB - Previously, we have reported that a 10-amino acid peptide (MIIYRDLISH) derived from the NH2-terminus of the human translationally controlled tumor protein (TCTP) functions as a protein transduction domain (PTD). In this study, we evaluated the transduction ability of SOD fused to TCTP-PTD (TCTP-SOD) into various cell lines. We also evaluated its ability to protect cells against paraquat-induced cell damage, in vitro and its neuroprotective effect in vivo against kainic acid-induced neuronal damage in an animal model. TCTP-SOD was transduced into various cell lines in a dose- and time-dependent manner without cytotoxic effect. Furthermore, TCTP-SOD showed cytoprotective activity in SH-SY5Y cells, and intraperitoneally, injected TCTP-SOD was delivered into the mouse brain and protected the cells in the hippocampal region against the damage induced by kainic acid. We propose TCTP-SOD as a potential candidate drug for treatment of brain diseases.
KW - Kainic acid
KW - Paraquat
KW - Protein transduction domain
KW - Superoxide dismutase
KW - Translationally controlled tumor protein
UR - http://www.scopus.com/inward/record.url?scp=79959964805&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2011.05.040
DO - 10.1016/j.ejphar.2011.05.040
M3 - Article
C2 - 21651901
AN - SCOPUS:79959964805
SN - 0014-2999
VL - 666
SP - 87
EP - 92
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1-3
ER -
