Neuroprotective effect of Cu,Zn-superoxide dismutase fused to a TCTP-derived protein transduction domain

Jisun Lee; Sabin Kim; Dong Hae Shin; Hwa Jung Kim; Kyunglim Lee

doi:10.1016/j.ejphar.2011.05.040

Neuroprotective effect of Cu,Zn-superoxide dismutase fused to a TCTP-derived protein transduction domain

Jisun Lee, Sabin Kim, Dong Hae Shin, Hwa Jung Kim, Kyunglim Lee

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Previously, we have reported that a 10-amino acid peptide (MIIYRDLISH) derived from the NH₂-terminus of the human translationally controlled tumor protein (TCTP) functions as a protein transduction domain (PTD). In this study, we evaluated the transduction ability of SOD fused to TCTP-PTD (TCTP-SOD) into various cell lines. We also evaluated its ability to protect cells against paraquat-induced cell damage, in vitro and its neuroprotective effect in vivo against kainic acid-induced neuronal damage in an animal model. TCTP-SOD was transduced into various cell lines in a dose- and time-dependent manner without cytotoxic effect. Furthermore, TCTP-SOD showed cytoprotective activity in SH-SY5Y cells, and intraperitoneally, injected TCTP-SOD was delivered into the mouse brain and protected the cells in the hippocampal region against the damage induced by kainic acid. We propose TCTP-SOD as a potential candidate drug for treatment of brain diseases.

Original language	English
Pages (from-to)	87-92
Number of pages	6
Journal	European Journal of Pharmacology
Volume	666
Issue number	1-3
DOIs	https://doi.org/10.1016/j.ejphar.2011.05.040
State	Published - Sep 2011

Bibliographical note

Funding Information:
This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs (A090030), NRF of Korea Grant funded by the Korean Government (2009–0064401), Mid-career Research Program through NRF grant funded by the MEST (R01-2007-000-20263-0) , Seoul R&BD Program (ST090801), and the NCRC program of MOST/KOSEF (R15-2006-020).

Keywords

Kainic acid
Paraquat
Protein transduction domain
Superoxide dismutase
Translationally controlled tumor protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ejphar.2011.05.040

Cite this

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title = "Neuroprotective effect of Cu,Zn-superoxide dismutase fused to a TCTP-derived protein transduction domain",

abstract = "Previously, we have reported that a 10-amino acid peptide (MIIYRDLISH) derived from the NH2-terminus of the human translationally controlled tumor protein (TCTP) functions as a protein transduction domain (PTD). In this study, we evaluated the transduction ability of SOD fused to TCTP-PTD (TCTP-SOD) into various cell lines. We also evaluated its ability to protect cells against paraquat-induced cell damage, in vitro and its neuroprotective effect in vivo against kainic acid-induced neuronal damage in an animal model. TCTP-SOD was transduced into various cell lines in a dose- and time-dependent manner without cytotoxic effect. Furthermore, TCTP-SOD showed cytoprotective activity in SH-SY5Y cells, and intraperitoneally, injected TCTP-SOD was delivered into the mouse brain and protected the cells in the hippocampal region against the damage induced by kainic acid. We propose TCTP-SOD as a potential candidate drug for treatment of brain diseases.",

keywords = "Kainic acid, Paraquat, Protein transduction domain, Superoxide dismutase, Translationally controlled tumor protein",

author = "Jisun Lee and Sabin Kim and Shin, {Dong Hae} and Kim, {Hwa Jung} and Kyunglim Lee",

note = "Funding Information: This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs (A090030), NRF of Korea Grant funded by the Korean Government (2009–0064401), Mid-career Research Program through NRF grant funded by the MEST (R01-2007-000-20263-0) , Seoul R&BD Program (ST090801), and the NCRC program of MOST/KOSEF (R15-2006-020).",

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AU - Shin, Dong Hae

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AU - Lee, Kyunglim

N1 - Funding Information: This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs (A090030), NRF of Korea Grant funded by the Korean Government (2009–0064401), Mid-career Research Program through NRF grant funded by the MEST (R01-2007-000-20263-0) , Seoul R&BD Program (ST090801), and the NCRC program of MOST/KOSEF (R15-2006-020).

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N2 - Previously, we have reported that a 10-amino acid peptide (MIIYRDLISH) derived from the NH2-terminus of the human translationally controlled tumor protein (TCTP) functions as a protein transduction domain (PTD). In this study, we evaluated the transduction ability of SOD fused to TCTP-PTD (TCTP-SOD) into various cell lines. We also evaluated its ability to protect cells against paraquat-induced cell damage, in vitro and its neuroprotective effect in vivo against kainic acid-induced neuronal damage in an animal model. TCTP-SOD was transduced into various cell lines in a dose- and time-dependent manner without cytotoxic effect. Furthermore, TCTP-SOD showed cytoprotective activity in SH-SY5Y cells, and intraperitoneally, injected TCTP-SOD was delivered into the mouse brain and protected the cells in the hippocampal region against the damage induced by kainic acid. We propose TCTP-SOD as a potential candidate drug for treatment of brain diseases.

AB - Previously, we have reported that a 10-amino acid peptide (MIIYRDLISH) derived from the NH2-terminus of the human translationally controlled tumor protein (TCTP) functions as a protein transduction domain (PTD). In this study, we evaluated the transduction ability of SOD fused to TCTP-PTD (TCTP-SOD) into various cell lines. We also evaluated its ability to protect cells against paraquat-induced cell damage, in vitro and its neuroprotective effect in vivo against kainic acid-induced neuronal damage in an animal model. TCTP-SOD was transduced into various cell lines in a dose- and time-dependent manner without cytotoxic effect. Furthermore, TCTP-SOD showed cytoprotective activity in SH-SY5Y cells, and intraperitoneally, injected TCTP-SOD was delivered into the mouse brain and protected the cells in the hippocampal region against the damage induced by kainic acid. We propose TCTP-SOD as a potential candidate drug for treatment of brain diseases.

KW - Kainic acid

KW - Paraquat

KW - Protein transduction domain

KW - Superoxide dismutase

KW - Translationally controlled tumor protein

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Neuroprotective effect of Cu,Zn-superoxide dismutase fused to a TCTP-derived protein transduction domain

Abstract

Bibliographical note

Keywords

UN SDGs

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