Neuroprotective effect of benzylideneacetophenone derivative on the MPTP model of neurodegeneration in mice

Jun Mo Kang, Jae Chul Jung, Heejeong Kim, Heena Lim, Soyong Jang, Seikwan Oh

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7 Scopus citations

Abstract

In this study, we investigated the neuroprotective effect of a benzylideneacetophenone derivative, JC3, in a mouse model of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD). C57BL/6 mice were treated with MPTP (30 mg/kg, i.p.) for 5 consecutive days. JC3 (10 mg/kg, i.p.) treatment was initiated 2 h after the first administration of MPTP and then at 24-h intervals for 3 consecutive days. The mice were sacrificed for analyses 7 days after the last MPTP injection. Immunohistochemistry and Western blot were used to determine the expression levels of tyrosine hydroxylase (TH), dopamine transporter (DAT), OX-42 (a marker of microglial activation), and glial fibrillary acid protein (GFAP, a marker of astrocyte activation) in the substantia nigra (SN) and striatum (ST). The results of these experiments demonstrated that JC3 restored the decreased TH-immunoreactivity (IR) and DAT and JC3 attenuated the increase in OX-42, GFAP, and COX-2 on the SN and ST on day 7 post-MPTP injection. These results suggest that JC3 can be a neuroprotective agent in an MPTP-induced model of PD.

Original languageEnglish
Pages (from-to)1098-1107
Number of pages10
JournalArchives of Pharmacal Research
Volume31
Issue number9
DOIs
StatePublished - Sep 2008

Keywords

  • Benzylideneacetophenone
  • DAT
  • GFAP
  • MPTP
  • OX42
  • Parkinson's disease

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