Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells

Ala Jo; June Sung; Sanghee Lee; Hyunsung Nam; Hyo Won Lee; Jongmin Park; Hwan Myung Kim; Eunha Kim; Seung Bum Park

doi:10.1021/acs.bioconjchem.8b00558

Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells

Ala Jo
, June Sung
, Sanghee Lee
, Hyunsung Nam
, Hyo Won Lee
, Jongmin Park
, Hwan Myung Kim
, Eunha Kim
, Seung Bum Park

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO₂H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with d/l-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO₂H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells.

Original language	English
Pages (from-to)	3394-3401
Number of pages	8
Journal	Bioconjugate Chemistry
Volume	29
Issue number	10
DOIs	https://doi.org/10.1021/acs.bioconjchem.8b00558
State	Published - 17 Oct 2018

Bibliographical note

Publisher Copyright:
Copyright © 2018 American Chemical Society.

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Cite this

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title = "Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells",

abstract = "Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO2H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with d/l-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO2H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells.",

author = "Ala Jo and June Sung and Sanghee Lee and Hyunsung Nam and Lee, \{Hyo Won\} and Jongmin Park and Kim, \{Hwan Myung\} and Eunha Kim and Park, \{Seung Bum\}",

note = "Publisher Copyright: Copyright {\textcopyright} 2018 American Chemical Society.",

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doi = "10.1021/acs.bioconjchem.8b00558",

language = "English",

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T1 - Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells

AU - Jo, Ala

AU - Sung, June

AU - Lee, Sanghee

AU - Nam, Hyunsung

AU - Lee, Hyo Won

AU - Park, Jongmin

AU - Kim, Hwan Myung

AU - Kim, Eunha

AU - Park, Seung Bum

PY - 2018/10/17

Y1 - 2018/10/17

N2 - Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO2H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with d/l-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO2H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells.

AB - Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO2H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with d/l-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO2H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells.

UR - https://www.scopus.com/pages/publications/85053601394

U2 - 10.1021/acs.bioconjchem.8b00558

DO - 10.1021/acs.bioconjchem.8b00558

M3 - Article

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AN - SCOPUS:85053601394

SN - 1043-1802

VL - 29

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EP - 3401

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 10

ER -

Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells

Abstract

Bibliographical note

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