Naphthalene-based fluorescent probes for glutathione and their applications in living cells and patients with sepsis

Jun Li, Younghee Kwon, Kyung Soo Chung, Chang Su Lim, Dayoung Lee, Yongkang Yue, Jisoo Yoon, Gyoungmi Kim, Sang Jip Nam, Youn Wokk Chung, Hwan Myung Kim, Caixia Yin, Ji Hwan Ryu, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Rationale: Among the biothiols-related diseases, sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection and can result in severe oxidative stress and damage to multiple organs. In this study, we aimed to develop a fluorescence chemosensor that can both detect GSH and further predict sepsis. Methods: In this study, two new naphthalene dialdehyde compounds containing different functional groups were synthesized, and the sensing abilities of these compounds towards biothiols and its applications for prediction of sepsis were investigated. Results: Our study revealed that the newly developed probe 6-methoxynaphthalene-2, 3-dicarbaldehyde (MNDA) has two-photon is capable of detecting GSH in live cells with two-photon microscopy (TPM) under the excitation at a wavelength of 900 nm. Furthermore, two GSH detection probes naphthalene-2,3-dicarboxaldehyde (NDA) and 6-fluoronaphthalene-2,3- dicarbaldehyde (FNDA) not only can detect GSH in living cells, but also showed clinical significance for the diagnosis and prediction of mortality in patients with sepsis. Conclusions: These results open up a promising direction for further medical diagnostic techniques.

Original languageEnglish
Pages (from-to)1411-1420
Number of pages10
JournalTheranostics
Volume8
Issue number5
DOIs
StatePublished - 2018

Keywords

  • Cell imaging
  • Diagnosis
  • Fluorescence probe
  • GSH
  • Sepsis

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