TY - JOUR
T1 - Naphthalamide-biotin-based fluorescent probe
T2 - A sensitive tool for CO detection in cancer cells and zebrafish
AU - Fortibui, Maxine Mambo
AU - Jang, Mina
AU - Roh, Jongtae
AU - Park, Yumi
AU - Maity, Souvik
AU - Ko, Sung Kyun
AU - Kim, Jinheung
N1 - Publisher Copyright:
© 2024
PY - 2024/10
Y1 - 2024/10
N2 - A fluorescent assay to detect carbon monoxide (CO), one of the signaling molecules in biological systems, was developed using a naphthalamide-biotin-based probe (EW5). The probe exhibited no significant fluorescence in phosphate-buffered saline/dimethyl sulfoxide solution. However, the fluorescence intensity of EW5 gradually increased at 533 nm with increasing concentrations of tricarbonyldichlororuthenium(II) dimer (CORM-2), a source of CO. Other possible competing analytes, such as Na+, K+, Fe2+, Zn2+, Cl−, NO3−, HS−, cysteine, homocysteine, glutathione, H2O2, O2. −, NO, t-BuOOH, NaOCl, and formaldehyde, exhibited no significant effect on the probe's emission intensity. Moreover, EW5's fluorescence intensity rapidly escalated, achieving equilibrium swiftly within 10 min, indicative of a prompt and effective response to carbon monoxide (CO). Additionally, EW5 demonstrated commendable photostability following 60 min of uninterrupted exposure to light. The data reveal that EW5 can discern CO presence in aqueous buffers, cancerous and living cells, as well as in zebrafish, with remarkable selectivity and steadfastness. It is distinctly present in lysosomes and mitochondria, offering a viable real-time marker for probing CO-related reactions in biological settings.
AB - A fluorescent assay to detect carbon monoxide (CO), one of the signaling molecules in biological systems, was developed using a naphthalamide-biotin-based probe (EW5). The probe exhibited no significant fluorescence in phosphate-buffered saline/dimethyl sulfoxide solution. However, the fluorescence intensity of EW5 gradually increased at 533 nm with increasing concentrations of tricarbonyldichlororuthenium(II) dimer (CORM-2), a source of CO. Other possible competing analytes, such as Na+, K+, Fe2+, Zn2+, Cl−, NO3−, HS−, cysteine, homocysteine, glutathione, H2O2, O2. −, NO, t-BuOOH, NaOCl, and formaldehyde, exhibited no significant effect on the probe's emission intensity. Moreover, EW5's fluorescence intensity rapidly escalated, achieving equilibrium swiftly within 10 min, indicative of a prompt and effective response to carbon monoxide (CO). Additionally, EW5 demonstrated commendable photostability following 60 min of uninterrupted exposure to light. The data reveal that EW5 can discern CO presence in aqueous buffers, cancerous and living cells, as well as in zebrafish, with remarkable selectivity and steadfastness. It is distinctly present in lysosomes and mitochondria, offering a viable real-time marker for probing CO-related reactions in biological settings.
KW - Carbon monoxide detection
KW - Cell imaging
KW - Fluorescent probe
KW - Naphthalamide biotin
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85195827776&partnerID=8YFLogxK
U2 - 10.1016/j.dyepig.2024.112278
DO - 10.1016/j.dyepig.2024.112278
M3 - Article
AN - SCOPUS:85195827776
SN - 0143-7208
VL - 229
JO - Dyes and Pigments
JF - Dyes and Pigments
M1 - 112278
ER -