Nanovesicle-Based Bioelectronic Nose for the Diagnosis of Lung Cancer from Human Blood

Jong Hyun Lim, Juhun Park, Eun Hae Oh, Hwi Jin Ko, Seunghun Hong, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

A human nose-mimetic diagnosis system that can distinguish the odor of a lung cancer biomarker, heptanal, from human blood is presented. Selective recognition of the biomarker is mimicked in the human olfactory system. A specific olfactory receptor recognizing the chemical biomarker is first selected through screening a library of human olfactory receptors (hORs). The selected hOR is expressed on the membrane of human embryonic kidney (HEK)-293 cells. Nanovesicles containing the hOR on the membrane are produced from these cells, and are then used for the functionalization of single-walled carbon nanotubes. This strategy allows the development of a sensitive and selective nanovesicle-based bioelectronic nose (NvBN). The NvBN is able to selectively detect heptanal at a concentration as low as 1 × 10-14 m, a sufficient level to distinguish the blood of a lung cancer patient from the blood of a healthy person. In actual experiments, NvBN could detect an extremely small increase in the amount of heptanal from human blood plasma without any pretreatment processes. This result offers a rapid and easy method to analyze chemical biomarkers from human blood in real-time and to diagnose lung cancer.

Original languageEnglish
Pages (from-to)360-366
Number of pages7
JournalAdvanced Healthcare Materials
Volume3
Issue number3
DOIs
StatePublished - Mar 2014

Keywords

  • Bioelectronic nose
  • Diagnosis
  • Heptanal
  • Lung cancer
  • Nanovesicles

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