Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation

Dokyoon Kim, Francesco Marchetti, Zuxiong Chen, Sasa Zaric, Robert J. Wilson, Drew A. Hall, Richard S. Gaster, Jung Rok Lee, Junyi Wang, Sebastian J. Osterfeld, Heng Yu, Robert M. White, William F. Blakely, Leif E. Peterson, Sandhya Bhatnagar, Brandon Mannion, Serena Tseng, Kristen Roth, Matthew Coleman, Antoine M. SnijdersAndrew J. Wyrobek, Shan X. Wang

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.

Original languageEnglish
Article number2234
JournalScientific Reports
Volume3
DOIs
StatePublished - 2013

Bibliographical note

Funding Information:
This project has been funded in whole or in part by Federal funds from the Biomedical Advanced Research and Development Authority, Office of the Assistant Secretary for Preparedness and Response, Office of the Secretary, Department of Health and Human Services, under Contract No. HHSO100201000006C. This work was performed under the auspices of the U.S. Department of Energy by the University of California, Lawrence Berkeley National Laboratory under DOE contract No. DE-AC02-05CH11231 and with funding support from BAA-BARDA-09-36. The Armed Forces Radiobiology Research Institute, under work units RAB4AP and RBB4AR, provided the support for one of the co-authors involved in this research (W.B.). The views expressed here are those of the authors; no endorsement by the U.S. Department of Defense or the U.S. government has been given or inferred. The work at Stanford also benefited from the National Cancer Institute grants Physical Science Oncology Center (U54CA143907), Center for Cancer Nanotechnology Excellence (U54CA151459), Innovative Molecular Analysis Technologies (R33CA138330).

Fingerprint

Dive into the research topics of 'Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation'. Together they form a unique fingerprint.

Cite this