Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation

Dokyoon Kim; Francesco Marchetti; Zuxiong Chen; Sasa Zaric; Robert J. Wilson; Drew A. Hall; Richard S. Gaster; Jung Rok Lee; Junyi Wang; Sebastian J. Osterfeld; Heng Yu; Robert M. White; William F. Blakely; Leif E. Peterson; Sandhya Bhatnagar; Brandon Mannion; Serena Tseng; Kristen Roth; Matthew Coleman; Antoine M. Snijders; Andrew J. Wyrobek; Shan X. Wang

doi:10.1038/srep02234

Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation

Dokyoon Kim, Francesco Marchetti, Zuxiong Chen, Sasa Zaric, Robert J. Wilson, Drew A. Hall, Richard S. Gaster, Jung Rok Lee, Junyi Wang, Sebastian J. Osterfeld, Heng Yu, Robert M. White, William F. Blakely, Leif E. Peterson, Sandhya Bhatnagar, Brandon Mannion, Serena Tseng, Kristen Roth, Matthew Coleman, Antoine M. SnijdersAndrew J. Wyrobek, Shan X. Wang

Department of Mechanical & Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.

Original language	English
Article number	2234
Journal	Scientific Reports
Volume	3
DOIs	https://doi.org/10.1038/srep02234
State	Published - 2013

Bibliographical note

Funding Information:
This project has been funded in whole or in part by Federal funds from the Biomedical Advanced Research and Development Authority, Office of the Assistant Secretary for Preparedness and Response, Office of the Secretary, Department of Health and Human Services, under Contract No. HHSO100201000006C. This work was performed under the auspices of the U.S. Department of Energy by the University of California, Lawrence Berkeley National Laboratory under DOE contract No. DE-AC02-05CH11231 and with funding support from BAA-BARDA-09-36. The Armed Forces Radiobiology Research Institute, under work units RAB4AP and RBB4AR, provided the support for one of the co-authors involved in this research (W.B.). The views expressed here are those of the authors; no endorsement by the U.S. Department of Defense or the U.S. government has been given or inferred. The work at Stanford also benefited from the National Cancer Institute grants Physical Science Oncology Center (U54CA143907), Center for Cancer Nanotechnology Excellence (U54CA151459), Innovative Molecular Analysis Technologies (R33CA138330).

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Kim, D., Marchetti, F., Chen, Z., Zaric, S., Wilson, R. J., Hall, D. A., Gaster, R. S., Lee, J. R., Wang, J., Osterfeld, S. J., Yu, H., White, R. M., Blakely, W. F., Peterson, L. E., Bhatnagar, S., Mannion, B., Tseng, S., Roth, K., Coleman, M., ... Wang, S. X. (2013). Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation. Scientific Reports, 3, Article 2234. https://doi.org/10.1038/srep02234

@article{05edc284680b48d5ab84c12821d452e3,

title = "Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation",

abstract = "Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.",

author = "Dokyoon Kim and Francesco Marchetti and Zuxiong Chen and Sasa Zaric and Wilson, {Robert J.} and Hall, {Drew A.} and Gaster, {Richard S.} and Lee, {Jung Rok} and Junyi Wang and Osterfeld, {Sebastian J.} and Heng Yu and White, {Robert M.} and Blakely, {William F.} and Peterson, {Leif E.} and Sandhya Bhatnagar and Brandon Mannion and Serena Tseng and Kristen Roth and Matthew Coleman and Snijders, {Antoine M.} and Wyrobek, {Andrew J.} and Wang, {Shan X.}",

note = "Funding Information: This project has been funded in whole or in part by Federal funds from the Biomedical Advanced Research and Development Authority, Office of the Assistant Secretary for Preparedness and Response, Office of the Secretary, Department of Health and Human Services, under Contract No. HHSO100201000006C. This work was performed under the auspices of the U.S. Department of Energy by the University of California, Lawrence Berkeley National Laboratory under DOE contract No. DE-AC02-05CH11231 and with funding support from BAA-BARDA-09-36. The Armed Forces Radiobiology Research Institute, under work units RAB4AP and RBB4AR, provided the support for one of the co-authors involved in this research (W.B.). The views expressed here are those of the authors; no endorsement by the U.S. Department of Defense or the U.S. government has been given or inferred. The work at Stanford also benefited from the National Cancer Institute grants Physical Science Oncology Center (U54CA143907), Center for Cancer Nanotechnology Excellence (U54CA151459), Innovative Molecular Analysis Technologies (R33CA138330).",

year = "2013",

doi = "10.1038/srep02234",

language = "English",

volume = "3",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

Kim, D, Marchetti, F, Chen, Z, Zaric, S, Wilson, RJ, Hall, DA, Gaster, RS, Lee, JR, Wang, J, Osterfeld, SJ, Yu, H, White, RM, Blakely, WF, Peterson, LE, Bhatnagar, S, Mannion, B, Tseng, S, Roth, K, Coleman, M, Snijders, AM, Wyrobek, AJ & Wang, SX 2013, 'Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation', Scientific Reports, vol. 3, 2234. https://doi.org/10.1038/srep02234

TY - JOUR

T1 - Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation

AU - Kim, Dokyoon

AU - Marchetti, Francesco

AU - Chen, Zuxiong

AU - Zaric, Sasa

AU - Wilson, Robert J.

AU - Hall, Drew A.

AU - Gaster, Richard S.

AU - Lee, Jung Rok

AU - Wang, Junyi

AU - Osterfeld, Sebastian J.

AU - Yu, Heng

AU - White, Robert M.

AU - Blakely, William F.

AU - Peterson, Leif E.

AU - Bhatnagar, Sandhya

AU - Mannion, Brandon

AU - Tseng, Serena

AU - Roth, Kristen

AU - Coleman, Matthew

AU - Snijders, Antoine M.

AU - Wyrobek, Andrew J.

AU - Wang, Shan X.

N1 - Funding Information: This project has been funded in whole or in part by Federal funds from the Biomedical Advanced Research and Development Authority, Office of the Assistant Secretary for Preparedness and Response, Office of the Secretary, Department of Health and Human Services, under Contract No. HHSO100201000006C. This work was performed under the auspices of the U.S. Department of Energy by the University of California, Lawrence Berkeley National Laboratory under DOE contract No. DE-AC02-05CH11231 and with funding support from BAA-BARDA-09-36. The Armed Forces Radiobiology Research Institute, under work units RAB4AP and RBB4AR, provided the support for one of the co-authors involved in this research (W.B.). The views expressed here are those of the authors; no endorsement by the U.S. Department of Defense or the U.S. government has been given or inferred. The work at Stanford also benefited from the National Cancer Institute grants Physical Science Oncology Center (U54CA143907), Center for Cancer Nanotechnology Excellence (U54CA151459), Innovative Molecular Analysis Technologies (R33CA138330).

PY - 2013

Y1 - 2013

N2 - Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.

AB - Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.

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U2 - 10.1038/srep02234

DO - 10.1038/srep02234

M3 - Article

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AN - SCOPUS:84880800169

SN - 2045-2322

VL - 3

JO - Scientific Reports

JF - Scientific Reports

M1 - 2234

ER -

Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation

Abstract

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