Nanoscopic monitroing of cytochrome c nitrosylation by plasmonic energy tranfer (PRET)

Sung S. Lee, Joe F. Lo, San Pun, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Label-free and real-time spectroscopic monitoring method for living cells is missing since current cellular imaging is based on fluorescent labeling, which cannot provide us fingerprints of molecules. We utilized Plasmon Resonance Energy Transfer (PRET)-based single-cell-level spectroscopy via nanoplasmonic probes (NPs) to differentiate the states of Cytochrome c (Cyt c) and monitor in-situ intracellular nitrosylation. The states were differentiated through quantized-dips within Rayleigh scattering spectrum of the NPs. Furthermore, endogenously nitrosylated Cyt c under apoptosis were measured by PRET. We expect this PRET-based detection has the potential to provide a novel platform to the fields of systems biology.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages302-304
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period1/11/095/11/09

Keywords

  • Label-free
  • Nanoplasmonic probes
  • Nitrosylation
  • Plasmon Resonance Energy Transfer (PRET)
  • Systems biology

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