Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics

Youngseop Lee, Byoung Hoon Kang, Minhee Kang, Doo Ryeon Chung, Gwan Su Yi, Luke P. Lee, Ki Hun Jeong

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Emerging molecular diagnosis requires ultrafast polymerase chain reaction (PCR) on chip for rapid precise detection of infectious diseases in the point-of-care test. Here, we report nanoplasmonic on-chip PCR for rapid precision molecular diagnostics. The nanoplasmonic pillar arrays (NPA) comprise gold nanoislands on the top and sidewall of large-scale glass nanopillar arrays. The nanoplasmonic pillars enhance light absorption of a white light-emitting diode (LED) over the whole visible range due to strong electromagnetic hotspots between the nanoislands. As a result, they effectively induce photothermal heating for ultrafast PCR thermal cycling. The temperature profile of NPA exhibits 30 cycles between 98 and 60 °C for a total of 3 min and 30 s during the cyclic excitation of white LED light. The experimental results also demonstrate the rapid DNA amplification of both 0.1 ng μL-1 of λ-DNA in 20 thermal cycles and 0.1 ng μL-1 of complementary DNA of Middle East respiratory syndrome coronavirus in 30 thermal cycles using a conventional PCR volume of 15 μL. This nanoplasmonic PCR technique provides a new opportunity for rapid precision molecular diagnostics.

Original languageEnglish
Pages (from-to)12533-12540
Number of pages8
JournalACS Applied Materials and Interfaces
Volume12
Issue number11
DOIs
StatePublished - 18 Mar 2020

Bibliographical note

Publisher Copyright:
© 2020 American Chemical Society.

Keywords

  • molecular diagnostics
  • nanopillar
  • photothermal effect
  • point-of-care diagnosis
  • polymerase chain reaction

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