Nanoplasmonic biomolecular ruler with a 2 NM spatial resolution for probing restricted digestion of nucleic acids

Gang L. Liu, Fanqing Chen, Daniele Gerion, Yadong Yin, A. Paul Alivisatos, Joe W. Gray, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

One of the major challenges of quantitative biochemistry and molecular biology is to monitor enzymatic activity within a femtoliter volume in real time. We have constructed a novel nanoscale plasmonic probe-based molecular ruler, which can perform label-free, real-time, and sensitive monitoring of DNA footprint or length during nuclease enzymatic reactions occurring around single nanoplasmonic biomolecular ruler. The nanoplasmonic molecular ruler was created by tethering specifically-designed double-stranded DNA to single Au nanoparticles. DNA length differences of as little as 2 nm (6 base pairs) after nuclease digestion are differentiated by the corresponding plasmon resonance shifts of the Au-DNA nanoconjugate.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1513-1515
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: 9 Oct 200513 Oct 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period9/10/0513/10/05

Keywords

  • Nanoparticles
  • Nuclease
  • Nucleic acids
  • Plasmon resonance

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