Nanogap-based dielectric-specific colocalization for highly sensitive surface plasmon resonance detection of biotin-streptavidin interactions

Yonghwi Kim; Kyungwha Chung; Wonju Lee; Dong Ha Kim; Donghyun Kim

doi:10.1063/1.4769108

Nanogap-based dielectric-specific colocalization for highly sensitive surface plasmon resonance detection of biotin-streptavidin interactions

Yonghwi Kim, Kyungwha Chung, Wonju Lee, Dong Ha Kim, Donghyun Kim

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We have performed highly sensitive surface plasmon resonance (SPR) detection by colocalizing the evanescent near-fields and target molecular distribution. The colocalization is based on oblique metal evaporation to form nanogaps of a size under 100 nm without using electron-beam lithography. The concept was demonstrated by detecting siloxane-based biotin/streptavidin interactions. 50-nm nanogaps produced the largest amplification of optical signatures and two orders of magnitude enhancement of sensitivity over conventional thin film-based measurements. The enhancement is associated with efficient overlap of localized near-fields and target. Colocalized detection scheme is expected to provide clues to molecular sensitivity for SPR biosensing.

Original language	English
Article number	233701
Journal	Applied Physics Letters
Volume	101
Issue number	23
DOIs	https://doi.org/10.1063/1.4769108
State	Published - 3 Dec 2012

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) grants funded by the Korean Government (2009-0070732, 2010-0007993, 2011-0017500, and 2011-0029409).

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title = "Nanogap-based dielectric-specific colocalization for highly sensitive surface plasmon resonance detection of biotin-streptavidin interactions",

abstract = "We have performed highly sensitive surface plasmon resonance (SPR) detection by colocalizing the evanescent near-fields and target molecular distribution. The colocalization is based on oblique metal evaporation to form nanogaps of a size under 100 nm without using electron-beam lithography. The concept was demonstrated by detecting siloxane-based biotin/streptavidin interactions. 50-nm nanogaps produced the largest amplification of optical signatures and two orders of magnitude enhancement of sensitivity over conventional thin film-based measurements. The enhancement is associated with efficient overlap of localized near-fields and target. Colocalized detection scheme is expected to provide clues to molecular sensitivity for SPR biosensing.",

author = "Yonghwi Kim and Kyungwha Chung and Wonju Lee and {Ha Kim}, Dong and Donghyun Kim",

note = "Funding Information: This work was supported by the National Research Foundation (NRF) grants funded by the Korean Government (2009-0070732, 2010-0007993, 2011-0017500, and 2011-0029409).",

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AU - Kim, Yonghwi

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AU - Lee, Wonju

AU - Ha Kim, Dong

AU - Kim, Donghyun

N1 - Funding Information: This work was supported by the National Research Foundation (NRF) grants funded by the Korean Government (2009-0070732, 2010-0007993, 2011-0017500, and 2011-0029409).

PY - 2012/12/3

Y1 - 2012/12/3

N2 - We have performed highly sensitive surface plasmon resonance (SPR) detection by colocalizing the evanescent near-fields and target molecular distribution. The colocalization is based on oblique metal evaporation to form nanogaps of a size under 100 nm without using electron-beam lithography. The concept was demonstrated by detecting siloxane-based biotin/streptavidin interactions. 50-nm nanogaps produced the largest amplification of optical signatures and two orders of magnitude enhancement of sensitivity over conventional thin film-based measurements. The enhancement is associated with efficient overlap of localized near-fields and target. Colocalized detection scheme is expected to provide clues to molecular sensitivity for SPR biosensing.

AB - We have performed highly sensitive surface plasmon resonance (SPR) detection by colocalizing the evanescent near-fields and target molecular distribution. The colocalization is based on oblique metal evaporation to form nanogaps of a size under 100 nm without using electron-beam lithography. The concept was demonstrated by detecting siloxane-based biotin/streptavidin interactions. 50-nm nanogaps produced the largest amplification of optical signatures and two orders of magnitude enhancement of sensitivity over conventional thin film-based measurements. The enhancement is associated with efficient overlap of localized near-fields and target. Colocalized detection scheme is expected to provide clues to molecular sensitivity for SPR biosensing.

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Nanogap-based dielectric-specific colocalization for highly sensitive surface plasmon resonance detection of biotin-streptavidin interactions

Abstract

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