Nanogap-based dielectric immunosensing

C. Di Carlo; Hokwan Kang; Xianghui Zeng; Ki Hun Jeong; L. P. Lee

doi:10.1109/SENSOR.2003.1216982

Nanogap-based dielectric immunosensing

C. Di Carlo, Hokwan Kang, Xianghui Zeng, Ki Hun Jeong, L. P. Lee

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

9 Scopus citations

Abstract

A novel device for measuring dielectric properties of protein bound to a surface without using a three-electrode system or redox couples is presented. This is achieved by reducing double layer capacitance contributions through decreased electrode gap size (60-100 nm). A proof of concept is demonstrated by immunosensing the glycoprotein laminin, which is clinically relevant to kidney disease at a concentration of 0.5 μg/ml. Dissipation factor measurements for different laminin concentrations indicate a detection limit of 10 ng/ml in a 30 microliter droplet. The measurement device described is applicable to a whole range of biological recognition systems including other protein-biomolecule binding interactions and nucleic acid hybridization.

Original language	English
Title of host publication	TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1180-1183
Number of pages	4
ISBN (Electronic)	0780377311, 9780780377318
DOIs	https://doi.org/10.1109/SENSOR.2003.1216982
State	Published - 2003
Event	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States Duration: 8 Jun 2003 → 12 Jun 2003

Publication series

Name	TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
Volume	2

Conference

Conference	12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
Country/Territory	United States
City	Boston
Period	8/06/03 → 12/06/03

Bibliographical note

Publisher Copyright:
© 2003 IEEE.

Keywords

Biomedical optical imaging
Biosensors
Capacitance
Dielectrics
Electrodes
Immune system
Nanobioscience
Optical sensors
Probes
Protein engineering

Access to Document

10.1109/SENSOR.2003.1216982

Cite this

Di Carlo, C., Kang, H., Zeng, X., Jeong, K. H., & Lee, L. P. (2003). Nanogap-based dielectric immunosensing. In TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers (pp. 1180-1183). Article 1216982 (TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers; Vol. 2). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/SENSOR.2003.1216982

Di Carlo, C. ; Kang, Hokwan ; Zeng, Xianghui et al. / Nanogap-based dielectric immunosensing. TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Institute of Electrical and Electronics Engineers Inc., 2003. pp. 1180-1183 (TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers).

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abstract = "A novel device for measuring dielectric properties of protein bound to a surface without using a three-electrode system or redox couples is presented. This is achieved by reducing double layer capacitance contributions through decreased electrode gap size (60-100 nm). A proof of concept is demonstrated by immunosensing the glycoprotein laminin, which is clinically relevant to kidney disease at a concentration of 0.5 μg/ml. Dissipation factor measurements for different laminin concentrations indicate a detection limit of 10 ng/ml in a 30 microliter droplet. The measurement device described is applicable to a whole range of biological recognition systems including other protein-biomolecule binding interactions and nucleic acid hybridization.",

keywords = "Biomedical optical imaging, Biosensors, Capacitance, Dielectrics, Electrodes, Immune system, Nanobioscience, Optical sensors, Probes, Protein engineering",

author = "{Di Carlo}, C. and Hokwan Kang and Xianghui Zeng and Jeong, {Ki Hun} and Lee, {L. P.}",

note = "Publisher Copyright: {\textcopyright} 2003 IEEE.; 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers ; Conference date: 08-06-2003 Through 12-06-2003",

year = "2003",

doi = "10.1109/SENSOR.2003.1216982",

language = "English",

series = "TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers",

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Di Carlo, C, Kang, H, Zeng, X, Jeong, KH & Lee, LP 2003, Nanogap-based dielectric immunosensing. in TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers., 1216982, TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers, vol. 2, Institute of Electrical and Electronics Engineers Inc., pp. 1180-1183, 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers, Boston, United States, 8/06/03. https://doi.org/10.1109/SENSOR.2003.1216982

Nanogap-based dielectric immunosensing. / Di Carlo, C.; Kang, Hokwan; Zeng, Xianghui et al.
TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Institute of Electrical and Electronics Engineers Inc., 2003. p. 1180-1183 1216982 (TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Nanogap-based dielectric immunosensing

AU - Di Carlo, C.

AU - Kang, Hokwan

AU - Zeng, Xianghui

AU - Jeong, Ki Hun

AU - Lee, L. P.

PY - 2003

Y1 - 2003

N2 - A novel device for measuring dielectric properties of protein bound to a surface without using a three-electrode system or redox couples is presented. This is achieved by reducing double layer capacitance contributions through decreased electrode gap size (60-100 nm). A proof of concept is demonstrated by immunosensing the glycoprotein laminin, which is clinically relevant to kidney disease at a concentration of 0.5 μg/ml. Dissipation factor measurements for different laminin concentrations indicate a detection limit of 10 ng/ml in a 30 microliter droplet. The measurement device described is applicable to a whole range of biological recognition systems including other protein-biomolecule binding interactions and nucleic acid hybridization.

AB - A novel device for measuring dielectric properties of protein bound to a surface without using a three-electrode system or redox couples is presented. This is achieved by reducing double layer capacitance contributions through decreased electrode gap size (60-100 nm). A proof of concept is demonstrated by immunosensing the glycoprotein laminin, which is clinically relevant to kidney disease at a concentration of 0.5 μg/ml. Dissipation factor measurements for different laminin concentrations indicate a detection limit of 10 ng/ml in a 30 microliter droplet. The measurement device described is applicable to a whole range of biological recognition systems including other protein-biomolecule binding interactions and nucleic acid hybridization.

KW - Biomedical optical imaging

KW - Biosensors

KW - Capacitance

KW - Dielectrics

KW - Electrodes

KW - Immune system

KW - Nanobioscience

KW - Optical sensors

KW - Probes

KW - Protein engineering

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U2 - 10.1109/SENSOR.2003.1216982

DO - 10.1109/SENSOR.2003.1216982

M3 - Conference contribution

AN - SCOPUS:84856647076

T3 - TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers

SP - 1180

EP - 1183

BT - TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers

Y2 - 8 June 2003 through 12 June 2003

ER -

Di Carlo C, Kang H, Zeng X, Jeong KH, Lee LP. Nanogap-based dielectric immunosensing. In TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Institute of Electrical and Electronics Engineers Inc. 2003. p. 1180-1183. 1216982. (TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers). doi: 10.1109/SENSOR.2003.1216982

Nanogap-based dielectric immunosensing

Abstract

Publication series

Conference

Bibliographical note

Keywords

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