Nanocavity network-based impedance sensor for molecular diagnostics

J. Tanner Nevill; Dino Di Carlo; Luke P. Lee

Nanocavity network-based impedance sensor for molecular diagnostics

J. Tanner Nevill, Dino Di Carlo, Luke P. Lee

Department of Chemistry & Nanoscience

Research output: Contribution to conference › Paper › peer-review

Abstract

We demonstrate label-free detection of a molecular binding event using a nanocavity network-based impedance technique. A micro-affinity column of biotinylated beads is packed inside of a glass microchip, and the binding of avidin occludes the nanocavities between beads and is measured as a change of impedance through the packed column. We developed a model that indicates a ∼8% increase in resistance upon saturated binding of avidin to the 1 μm diameter beads in the column. Preliminary data in 0.84 μm beads indicates a 20% increase in resistance upon saturation with avidin.

Original language	English
Pages	1283-1285
Number of pages	3
State	Published - 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 5 Nov 2006 → 9 Nov 2006

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	5/11/06 → 9/11/06

Keywords

Bead-based analysis
Impedance sensor
Label-free
Microparticles

Cite this

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title = "Nanocavity network-based impedance sensor for molecular diagnostics",

abstract = "We demonstrate label-free detection of a molecular binding event using a nanocavity network-based impedance technique. A micro-affinity column of biotinylated beads is packed inside of a glass microchip, and the binding of avidin occludes the nanocavities between beads and is measured as a change of impedance through the packed column. We developed a model that indicates a ∼8% increase in resistance upon saturated binding of avidin to the 1 μm diameter beads in the column. Preliminary data in 0.84 μm beads indicates a 20% increase in resistance upon saturation with avidin.",

keywords = "Bead-based analysis, Impedance sensor, Label-free, Microparticles",

author = "Nevill, {J. Tanner} and {Di Carlo}, Dino and Lee, {Luke P.}",

year = "2006",

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note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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T1 - Nanocavity network-based impedance sensor for molecular diagnostics

AU - Nevill, J. Tanner

AU - Di Carlo, Dino

AU - Lee, Luke P.

PY - 2006

Y1 - 2006

N2 - We demonstrate label-free detection of a molecular binding event using a nanocavity network-based impedance technique. A micro-affinity column of biotinylated beads is packed inside of a glass microchip, and the binding of avidin occludes the nanocavities between beads and is measured as a change of impedance through the packed column. We developed a model that indicates a ∼8% increase in resistance upon saturated binding of avidin to the 1 μm diameter beads in the column. Preliminary data in 0.84 μm beads indicates a 20% increase in resistance upon saturation with avidin.

AB - We demonstrate label-free detection of a molecular binding event using a nanocavity network-based impedance technique. A micro-affinity column of biotinylated beads is packed inside of a glass microchip, and the binding of avidin occludes the nanocavities between beads and is measured as a change of impedance through the packed column. We developed a model that indicates a ∼8% increase in resistance upon saturated binding of avidin to the 1 μm diameter beads in the column. Preliminary data in 0.84 μm beads indicates a 20% increase in resistance upon saturation with avidin.

KW - Bead-based analysis

KW - Impedance sensor

KW - Label-free

KW - Microparticles

UR - http://www.scopus.com/inward/record.url?scp=84902438652&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902438652

SP - 1283

EP - 1285

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Nanocavity network-based impedance sensor for molecular diagnostics

Abstract

Conference

Keywords

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Cite this