N-Methyl-d-Aspartate Receptor-Mediated Chemotaxis and Ca2+ Signaling in Tetrahymena pyriformis

Seong Won Nam, Sang Tae Kim, Kang Mu Lee, So Hyun Kim, Songzi Kou, Jeesun Lim, Hyejin Hwang, Min Kyung Joo, Byeongmoon Jeong, Seung Hyun Yoo, Sungsu Park

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Although the ciliate Tetrahymena is a good model for the study of chemotaxis, its profound motility makes it difficult to monitor intracellular calcium (Ca2+) changes induced by chemotactic stimuli. In this study, we report a microfluidic-based chemotaxis system generating directional chemotactic gradients under highly viscous conditions, suppressing T. pyriformis motility, and allowing for the stable confocal imaging of changes in intracellular Ca2+ in the ciliate. Once the viscous condition was achieved, directional chemical gradients were formed inside the center chamber via the release of N-methyl-d-aspartate (NMDA), a known chemoattractant, from the surrounding chemical reservoirs into the center chamber. As a result, intracellular Ca2+ in the ciliate increased up to three-fold, and its distribution was skewed in the direction of NMDA stimulation. However, the Ca2+ in ciliates pretreated with phospholipase C (PLC) or phosphatidylinositol-3-kinase (PI3K) blockers did not increase even after stimulation. Additionally, the PI3K blocker induced the secretion of granules, the size of which was dependent on the concentration of the blocker. Collectively, the results indicate that both PLC and PI3K perform pivotal roles in controlling the levels of intracellular Ca2+ in T. pyriformis during chemotaxis.

Original languageEnglish
Pages (from-to)331-342
Number of pages12
JournalProtist
Volume160
Issue number2
DOIs
StatePublished - May 2009

Keywords

  • Ca signaling
  • NMDA receptor
  • Tetrahymena
  • granules
  • microfluidics
  • motility

Fingerprint

Dive into the research topics of 'N-Methyl-d-Aspartate Receptor-Mediated Chemotaxis and Ca2+ Signaling in Tetrahymena pyriformis'. Together they form a unique fingerprint.

Cite this