Multiplexed Ultrasensitive Sample-to-Answer RT-LAMP Chip for the Identification of SARS-CoV-2 and Influenza Viruses

Minsun Song, Soon Gweon Hong, Luke P. Lee

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Prompt on-site diagnosis of SARS-CoV-2 with other respiratory infections will have minimized the global impact of the COVID-19 pandemic through rapid, effective management. However, no such multiplex point-of-care (POC) chip has satisfied a suitable sensitivity of gold-standard nucleic acid amplification tests (NAATs). Here, a rapid multiplexed ultrasensitive sample-to-answer loop-mediated isothermal amplification (MUSAL) chip operated by simple LED-driven photothermal amplification to detect six targets from single-swab sampling is presented. First, the MUSAL chip allows ultrafast on-chip sample preparation with ≈500-fold preconcentration at a rate of 1.2 mL min−1. Second, the chip enables contamination-free amplification using autonomous target elution into on-chip reagents by photothermal activation. Finally, the chip accomplishes multiplexed on-chip diagnostics of SARS-CoV-2 and influenza viruses with a limit of detection (LoD) of 0.5 copies µL−1. The rapid, ultrasensitive, cost-effective sample-to-answer chip with a multiplex capability will allow timely management of various pandemics situations that may be faced shortly.

Original languageEnglish
Article number2207138
JournalAdvanced Materials
Volume35
Issue number10
DOIs
StatePublished - 9 Mar 2023

Bibliographical note

Publisher Copyright:
© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.

Keywords

  • SARS-CoV-2
  • multiplexing
  • on-chip sample preparation
  • preconcentration
  • reverse transcription loop-mediated isothermal amplification
  • sample-to-answer
  • ultrasensitive point-of-care chips

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