Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher

Xinglu Huang; Magdalena Swierczewska; Ki Young Choi; Lei Zhu; Ashwinkumar Bhirde; Jinwoo Park; Kwangmeyung Kim; Jin Xie; Gang Niu; Kang Choon Lee; Seulki Lee; Xiaoyuan Chen

doi:10.1002/anie.201107795

Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher

Xinglu Huang, Magdalena Swierczewska, Ki Young Choi, Lei Zhu, Ashwinkumar Bhirde, Jinwoo Park, Kwangmeyung Kim, Jin Xie, Gang Niu, Kang Choon Lee, Seulki Lee, Xiaoyuan Chen

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).

Original language	English
Pages (from-to)	1625-1630
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	7
DOIs	https://doi.org/10.1002/anie.201107795
State	Published - 13 Feb 2012

Keywords

caspases
fluorescence
imaging agents
nanoparticles
proteolysis

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10.1002/anie.201107795

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abstract = "Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).",

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AU - Huang, Xinglu

AU - Swierczewska, Magdalena

AU - Choi, Ki Young

AU - Zhu, Lei

AU - Bhirde, Ashwinkumar

AU - Park, Jinwoo

AU - Kim, Kwangmeyung

AU - Xie, Jin

AU - Niu, Gang

AU - Lee, Kang Choon

AU - Lee, Seulki

AU - Chen, Xiaoyuan

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KW - imaging agents

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Multiplex imaging of an intracellular proteolytic cascade by using a broad-spectrum nanoquencher

Abstract

Keywords

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