Multimerization of the cytoplasmic domain of syndecan-4 is required for its ability to activate protein kinase C

Eok Soo Oh, Anne Woods, John R. Couchman

Research output: Contribution to journalArticlepeer-review

188 Scopus citations


The transmembrane proteoglycan syndecan-4, which is a coreceptor with integrins in cytoskeleton-matrix interactions, appears to be multimerized in vivo. Both purified and recombinant core proteins form sodium dodecyl sulfate-resistant oligomers, and we now report that a synthetic peptide corresponding to the central region of syndecan-4 cytoplasmic domain (4V) also oligomerizes. The degree of oligomerization correlates with the previously reported ability to bind protein kinase C (PKC) and regulate its activity. Only multimeric recombinant syndecan-4 core protein, but not the monomeric protein, potentiated the activity of PKCα, and only oligomeric syndecan-4 cytoplasmic peptides were active. Changes in peptide sequence caused parallel loss of stable oligomeric status and ability to regulate a mixture of PKCαβγ activity. A synthetic peptide encompassing the whole cytoplasmic domain of syndecan-4 (4L) containing a membrane-proximal basic sequence did not form higher order oligomers and could not regulate the activity of PKCαβγ, unless induced to aggregate by phosphatidylinositol 4,5-bisphosphate. Oligomerization and PKC regulatory activity of the 4V peptide were both increased by addition of N-terminal cysteine and reduced by phosphorylation of the cysteine thiol group. Concentration of syndecan-4 at sites of focal adhesion formation may enhance multimerization and both localize PKC and potentiate its activity to induce stable complex formation.

Original languageEnglish
Pages (from-to)11805-11811
Number of pages7
JournalJournal of Biological Chemistry
Issue number18
StatePublished - 2 May 1997


Dive into the research topics of 'Multimerization of the cytoplasmic domain of syndecan-4 is required for its ability to activate protein kinase C'. Together they form a unique fingerprint.

Cite this