Multifunctional nanoparticles for gene delivery and spinal cord injury

So Jung Gwak, Heebeom Koo, Yeomin Yun, Ji Young Yhee, Hye Yeong Lee, Do Heum Yoon, Kwangmeyung Kim, Yoon Ha

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Methylprednisolone (MP) is a glucocorticoid that is used as an anti-inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was used to synthesize chitosan-MP conjugate, which was used to evaluate the gene therapy, anti-inflammatory and anti-apoptotic effects of MP. The cytotoxicity of chitosan-MP nanoparticles and the transfection efficiency of plasmid DNA were evaluated by MTT and luciferase assays. A chitosan-MP/pDNA complexes was injected into injured spinal cord to evaluate the anti-inflammatory and anti-apoptotic effects of these complexes using terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) and ED1 staining, respectively. In addition, to evaluate the distribution of chitosan-MP/pDNA complexes, pβ-gal encapsulated chitosan-MP was injected into the injected site. Cell survival was similar in cells treated with chitosan-MP conjugate and untreated cells. Luciferase expression was higher in cells treated with the chitosan-MP/pDNA than cells treated with the chitosan/pDNA. The chitosan-MP/pDNA complexes also reduced apoptosis and inflammation at the injury site. These results suggest that chitosan-MP conjugation is an effective gene delivery system to treat SCI.

Original languageEnglish
Pages (from-to)3474-3482
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Issue number11
StatePublished - 1 Nov 2015

Bibliographical note

Publisher Copyright:
© 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3474-3482, 2015. © 2015 Wiley Periodicals, Inc.


  • chitosan
  • gene delivery
  • methylprednisolone
  • spinal cord injury


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