Multi-wavelength light emitting diode-based disposable optrode array for in vivo optogenetic modulation

Saeyeong Jeon, Jae Hyun Kim, Hongkyun Lee, Yong Kweon Kim, Sang Beom Jun, Seung Hee Lee, Chang Hyeon Ji

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


We present a light emitting diode (LED)-based optical waveguide array that can optogenetically modulate genetically targeted neurons in the brain. The reusable part of the system consists of control electronics and conventional multi-wavelength LED. The disposable part comprises optical fibers assembled with microlens array fabricated on a silicon die. Both parts can be easily assembled and separated by snap fit structure. Measured light intensity is 3.35 mW/mm2 at 469 nm and 0.29 mW/mm2 at 590 nm when the applied current is 80 mA. In all the tested conditions, the light-induced temperature rise is under 0.5 C and over 90% of the relative light intensity is maintained at 2 mm-distance from the fiber tips. We further tested the efficiency of the optical array in vivo at 469 nm. When the optical array delivers light stimulation on to the visual cortex of a mouse expressing channelrhodopsin-2, the neural activity is significantly increased. The light-driven neural activity is successfully transformed into a percept of the mouse, showing significant learning of the task detecting the cortical stimulation. Our results demonstrate that the proposed optical array interfaces well with the neural circuits in vivo and the system is applicable to guide animal behaviors.

Original languageEnglish
Article numbere201800343
JournalJournal of Biophotonics
Issue number5
StatePublished - May 2019

Bibliographical note

Funding Information:
Ministry of Science, ICT & Future Planning, Grant/Award Numbers: CISS-2012M3A6A6054204, CISS-2016M3A6A6930773; National Research Foundation of Korea, Grant/Award Numbers: 2015R1D1A1A09058627, 2018M3C1B8016147.

Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


  • Implanted neurostimulators
  • Neuroscience
  • Optical fibers
  • Optogenetics


Dive into the research topics of 'Multi-wavelength light emitting diode-based disposable optrode array for in vivo optogenetic modulation'. Together they form a unique fingerprint.

Cite this