Mulberry Fruit Extract Ameliorates Adipogenesis via Increasing AMPK Activity and Downregulating MicroRNA-21/143 in 3T3-L1 Adipocytes

Mak Soon Lee, Yangha Kim

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Mulberry (Morus alba L.) fruits have long been used in traditional medicine and as edible berries in many countries. This study investigated the antiadipogenic effect of high hydrostatic pressure mulberry fruit extract (MFE) during 3T3-L1 adipocyte differentiation. MFE decreased lipid and triglyceride accumulation and glycerol-3-phosphate dehydrogenase activity. The mRNA expression levels of genes related to adipogenesis, such as the adipocyte protein 2, proliferator-activated receptor-γ, and CCAAT/enhancer binding protein-α, were suppressed by MFE. They also reduced microRNA (miR)-21 and miR-143 expression, which are involved in adipogenesis. In contrast, adenosine monophosphate-activated protein kinase (AMPK) activity was increased by MFE. These results suggested that MFE may suppress adipogenesis through modulating miR-21/143 expression and AMPK activity in 3T3-L1 adipocytes, which may be useful as antiobesity food agents.

Original languageEnglish
Pages (from-to)266-272
Number of pages7
JournalJournal of Medicinal Food
Volume23
Issue number3
DOIs
StatePublished - Mar 2020

Bibliographical note

Publisher Copyright:
© Mary Ann Liebert, Inc., publishers, and Korean Society of Food Science and Nutrition 2020.

Keywords

  • AMPK
  • adipogenesis
  • microRNA-21/143
  • mulberry fruit extract

Fingerprint

Dive into the research topics of 'Mulberry Fruit Extract Ameliorates Adipogenesis via Increasing AMPK Activity and Downregulating MicroRNA-21/143 in 3T3-L1 Adipocytes'. Together they form a unique fingerprint.

Cite this