Molecular profiling of single circulating tumor cells from lung cancer patients

Seung Min Park; Dawson J. Wong; Chin Chun Ooi; David M. Kurtz; Ophir Vermesh; Amin Aalipour; Susie Suh; Kelsey L. Pian; Jacob J. Chabon; Sang Hun Lee; Mehran Jamali; Carmen Say; Justin N. Carter; Luke P. Lee; Ware G. Kuschner; Erich J. Schwartz; Joseph B. Shrager; Joel W. Neal; Heather A. Wakelee; Maximilian Diehn; Viswam S. Nair; Shan X. Wang; Sanjiv S. Gambhir

doi:10.1073/pnas.1608461113

Molecular profiling of single circulating tumor cells from lung cancer patients

Seung Min Park, Dawson J. Wong, Chin Chun Ooi, David M. Kurtz, Ophir Vermesh, Amin Aalipour, Susie Suh, Kelsey L. Pian, Jacob J. Chabon, Sang Hun Lee, Mehran Jamali, Carmen Say, Justin N. Carter, Luke P. Lee, Ware G. Kuschner, Erich J. Schwartz, Joseph B. Shrager, Joel W. Neal, Heather A. Wakelee, Maximilian DiehnViswam S. Nair, Shan X. Wang, Sanjiv S. Gambhir

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

Circulating tumor cells (CTCs) are established cancer biomarkers for the "liquid biopsy" of tumors. Molecular analysis of single CTCs, which recapitulate primary and metastatic tumor biology, remains challenging because current platforms have limited throughput, are expensive, and are not easily translatable to the clinic. Here, we report a massively parallel, multigene-profiling nanoplatform to compartmentalize and analyze hundreds of single CTCs. After high-efficiency magnetic collection of CTC from blood, a single-cell nanowell array performs CTC mutation profiling using modular gene panels. Using this approach, we demonstrated multigene expression profiling of individual CTCs from non-small-cell lung cancer (NSCLC) patients with remarkable sensitivity. Thus, we report a highthroughput, multiplexed strategy for single-cell mutation profiling of individual lung cancer CTCs toward minimally invasive cancer therapy prediction and disease monitoring.

Original language	English
Pages (from-to)	E8379-E8386
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	113
Issue number	52
DOIs	https://doi.org/10.1073/pnas.1608461113
State	Published - 27 Dec 2016

Keywords

Circulating tumor cells
Microfluidics
Rare-cell sorting
Reverse transcription-PCR
Single-cell analysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1073/pnas.1608461113

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Park, S. M., Wong, D. J., Ooi, C. C., Kurtz, D. M., Vermesh, O., Aalipour, A., Suh, S., Pian, K. L., Chabon, J. J., Lee, S. H., Jamali, M., Say, C., Carter, J. N., Lee, L. P., Kuschner, W. G., Schwartz, E. J., Shrager, J. B., Neal, J. W., Wakelee, H. A., ... Gambhir, S. S. (2016). Molecular profiling of single circulating tumor cells from lung cancer patients. Proceedings of the National Academy of Sciences of the United States of America, 113(52), E8379-E8386. https://doi.org/10.1073/pnas.1608461113

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title = "Molecular profiling of single circulating tumor cells from lung cancer patients",

abstract = "Circulating tumor cells (CTCs) are established cancer biomarkers for the {"}liquid biopsy{"} of tumors. Molecular analysis of single CTCs, which recapitulate primary and metastatic tumor biology, remains challenging because current platforms have limited throughput, are expensive, and are not easily translatable to the clinic. Here, we report a massively parallel, multigene-profiling nanoplatform to compartmentalize and analyze hundreds of single CTCs. After high-efficiency magnetic collection of CTC from blood, a single-cell nanowell array performs CTC mutation profiling using modular gene panels. Using this approach, we demonstrated multigene expression profiling of individual CTCs from non-small-cell lung cancer (NSCLC) patients with remarkable sensitivity. Thus, we report a highthroughput, multiplexed strategy for single-cell mutation profiling of individual lung cancer CTCs toward minimally invasive cancer therapy prediction and disease monitoring.",

keywords = "Circulating tumor cells, Microfluidics, Rare-cell sorting, Reverse transcription-PCR, Single-cell analysis",

author = "Park, {Seung Min} and Wong, {Dawson J.} and Ooi, {Chin Chun} and Kurtz, {David M.} and Ophir Vermesh and Amin Aalipour and Susie Suh and Pian, {Kelsey L.} and Chabon, {Jacob J.} and Lee, {Sang Hun} and Mehran Jamali and Carmen Say and Carter, {Justin N.} and Lee, {Luke P.} and Kuschner, {Ware G.} and Schwartz, {Erich J.} and Shrager, {Joseph B.} and Neal, {Joel W.} and Wakelee, {Heather A.} and Maximilian Diehn and Nair, {Viswam S.} and Wang, {Shan X.} and Gambhir, {Sanjiv S.}",

year = "2016",

month = dec,

day = "27",

doi = "10.1073/pnas.1608461113",

language = "English",

volume = "113",

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Park, SM, Wong, DJ, Ooi, CC, Kurtz, DM, Vermesh, O, Aalipour, A, Suh, S, Pian, KL, Chabon, JJ, Lee, SH, Jamali, M, Say, C, Carter, JN, Lee, LP, Kuschner, WG, Schwartz, EJ, Shrager, JB, Neal, JW, Wakelee, HA, Diehn, M, Nair, VS, Wang, SX & Gambhir, SS 2016, 'Molecular profiling of single circulating tumor cells from lung cancer patients', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 52, pp. E8379-E8386. https://doi.org/10.1073/pnas.1608461113

TY - JOUR

T1 - Molecular profiling of single circulating tumor cells from lung cancer patients

AU - Park, Seung Min

AU - Wong, Dawson J.

AU - Ooi, Chin Chun

AU - Kurtz, David M.

AU - Vermesh, Ophir

AU - Aalipour, Amin

AU - Suh, Susie

AU - Pian, Kelsey L.

AU - Chabon, Jacob J.

AU - Lee, Sang Hun

AU - Jamali, Mehran

AU - Say, Carmen

AU - Carter, Justin N.

AU - Lee, Luke P.

AU - Kuschner, Ware G.

AU - Schwartz, Erich J.

AU - Shrager, Joseph B.

AU - Neal, Joel W.

AU - Wakelee, Heather A.

AU - Diehn, Maximilian

AU - Nair, Viswam S.

AU - Wang, Shan X.

AU - Gambhir, Sanjiv S.

PY - 2016/12/27

Y1 - 2016/12/27

N2 - Circulating tumor cells (CTCs) are established cancer biomarkers for the "liquid biopsy" of tumors. Molecular analysis of single CTCs, which recapitulate primary and metastatic tumor biology, remains challenging because current platforms have limited throughput, are expensive, and are not easily translatable to the clinic. Here, we report a massively parallel, multigene-profiling nanoplatform to compartmentalize and analyze hundreds of single CTCs. After high-efficiency magnetic collection of CTC from blood, a single-cell nanowell array performs CTC mutation profiling using modular gene panels. Using this approach, we demonstrated multigene expression profiling of individual CTCs from non-small-cell lung cancer (NSCLC) patients with remarkable sensitivity. Thus, we report a highthroughput, multiplexed strategy for single-cell mutation profiling of individual lung cancer CTCs toward minimally invasive cancer therapy prediction and disease monitoring.

AB - Circulating tumor cells (CTCs) are established cancer biomarkers for the "liquid biopsy" of tumors. Molecular analysis of single CTCs, which recapitulate primary and metastatic tumor biology, remains challenging because current platforms have limited throughput, are expensive, and are not easily translatable to the clinic. Here, we report a massively parallel, multigene-profiling nanoplatform to compartmentalize and analyze hundreds of single CTCs. After high-efficiency magnetic collection of CTC from blood, a single-cell nanowell array performs CTC mutation profiling using modular gene panels. Using this approach, we demonstrated multigene expression profiling of individual CTCs from non-small-cell lung cancer (NSCLC) patients with remarkable sensitivity. Thus, we report a highthroughput, multiplexed strategy for single-cell mutation profiling of individual lung cancer CTCs toward minimally invasive cancer therapy prediction and disease monitoring.

KW - Circulating tumor cells

KW - Microfluidics

KW - Rare-cell sorting

KW - Reverse transcription-PCR

KW - Single-cell analysis

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U2 - 10.1073/pnas.1608461113

DO - 10.1073/pnas.1608461113

M3 - Article

C2 - 27956614

AN - SCOPUS:85007494746

SN - 0027-8424

VL - 113

SP - E8379-E8386

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 52

ER -

Molecular profiling of single circulating tumor cells from lung cancer patients

Abstract

Keywords

UN SDGs

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