Molecular overlap with optical near-fields based on plasmonic nanolithography for ultrasensitive label-free detection by light-matter colocalization

Kiheung Kim, Wonju Lee, Kyungwha Chung, Hongki Lee, Taehwang Son, Youngjin Oh, Yun Feng Xiao, Dong Ha Kim, Donghyun Kim

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

In this work, we investigate the detection sensitivity of surface plasmon resonance (SPR) biosensors by engineering spatial distribution of electromagnetic near-fields for colocalization with molecular distribution. The light-matter colocalization was based on plasmonic nanolithography, the concept of which was confirmed by detecting streptavidin biotin interactions on triangular nanoaperture arrays after the structure of the aperture arrays was optimized for colocalization efficiency. The colocalization was shown to amplify optical signature significantly and thereby to achieve detection on the order of 100 streptavidin molecules with a binding capacity below 1 fg/mm2, an enhancement by more than three orders of magnitude over conventional SPR detection.

Original languageEnglish
Pages (from-to)89-98
Number of pages10
JournalBiosensors and Bioelectronics
Volume96
DOIs
StatePublished - 15 Oct 2017

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) Grants (NRF-2012R1A4A1029061, NRF-2015R1A2A1A10052826,and NRF-2016K2A9A2A06004899). K. Chung and D.H. Kim acknowledge the financial support by the NRF of Korea Grant funded by the Korean Government (2014R1A2A1A09005656).

Publisher Copyright:
© 2017 Elsevier B.V.

Keywords

  • Biosensor
  • Colocalization
  • Plasmonic lithography
  • Sensitivity
  • Surface plasmon resonance

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