Abstract
It is well established that growth-factor-induced reactive oxygen species (ROS) act as second messengers in cell signaling. We have previously reported that βPix, a guanine nucleotide exchange factor for Rac, interacts with NADPH oxidase 1 (Nox1) leading to EGF-induced ROS generation. Here, we report the identification of the domains of Nox1 and βPix responsible for the interaction between the two proteins. GST pull-down assays show that the PH domain of βPix binds to the FAD-binding region of Nox1. We also show that overexpression of the PH domain of βPix results in inhibition of superoxide anion generation in response to EGF. Additionally, NADPH oxidase Organizer 1 (NoxO1) is shown to interact with the NADPH-binding region of Nox1. These results suggest that the formation of the complex consisting of Nox1, βPix, and NoxO1 is likely to be a critical step in EGF-induced ROS generation.
Original language | English |
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Pages (from-to) | 985-990 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 339 |
Issue number | 3 |
DOIs | |
State | Published - 20 Jan 2006 |
Keywords
- βPix
- EGF
- NADPH oxidase 1
- NADPH oxidase Organizer 1
- Reactive oxygen species
- Superoxide anion