Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)

Dong Uk Kim; Tae Young Roh; Jieun Lee; Joo Yoon Noh; Young Joo Jang; Kwang Lae Hoe; Hyang Sook Yoo; Myung Un Choi

doi:10.1016/S1388-1981(99)00020-7

Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)

Dong Uk Kim, Tae Young Roh, Jieun Lee, Joo Yoon Noh, Young Joo Jang, Kwang Lae Hoe, Hyang Sook Yoo, Myung Un Choi

Department of Life Science

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino- acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.

Original language	English
Pages (from-to)	409-414
Number of pages	6
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	1437
Issue number	3
DOIs	https://doi.org/10.1016/S1388-1981(99)00020-7
State	Published - 25 Mar 1999

Bibliographical note

Funding Information:
This work was supported by the Ministry of Education, Republic of Korea (BSR1-96-3418), in part by the Korea Science and Engineering Foundation (KOSEF) through the Center for Molecular Catalysis at Seoul National University, and by the Ministry of Science and Technology of Korea (Grants KG1012, KG1091, KS1011, and KS1090).

Keywords

(Brassica oleracea var. capitata)
cDNA sequence
Expression
Phospholipase D

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10.1016/S1388-1981(99)00020-7

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title = "Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)",

abstract = "We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino- acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.",

keywords = "(Brassica oleracea var. capitata), cDNA sequence, Expression, Phospholipase D",

author = "Kim, {Dong Uk} and Roh, {Tae Young} and Jieun Lee and Noh, {Joo Yoon} and Jang, {Young Joo} and Hoe, {Kwang Lae} and Yoo, {Hyang Sook} and Choi, {Myung Un}",

note = "Funding Information: This work was supported by the Ministry of Education, Republic of Korea (BSR1-96-3418), in part by the Korea Science and Engineering Foundation (KOSEF) through the Center for Molecular Catalysis at Seoul National University, and by the Ministry of Science and Technology of Korea (Grants KG1012, KG1091, KS1011, and KS1090).",

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doi = "10.1016/S1388-1981(99)00020-7",

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T1 - Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)

AU - Kim, Dong Uk

AU - Roh, Tae Young

AU - Lee, Jieun

AU - Noh, Joo Yoon

AU - Jang, Young Joo

AU - Hoe, Kwang Lae

AU - Yoo, Hyang Sook

AU - Choi, Myung Un

N1 - Funding Information: This work was supported by the Ministry of Education, Republic of Korea (BSR1-96-3418), in part by the Korea Science and Engineering Foundation (KOSEF) through the Center for Molecular Catalysis at Seoul National University, and by the Ministry of Science and Technology of Korea (Grants KG1012, KG1091, KS1011, and KS1090).

PY - 1999/3/25

Y1 - 1999/3/25

N2 - We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino- acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.

AB - We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino- acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.

KW - (Brassica oleracea var. capitata)

KW - cDNA sequence

KW - Expression

KW - Phospholipase D

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U2 - 10.1016/S1388-1981(99)00020-7

DO - 10.1016/S1388-1981(99)00020-7

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AN - SCOPUS:0033602396

SN - 1388-1981

VL - 1437

SP - 409

EP - 414

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

IS - 3

ER -

Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)

Abstract

Bibliographical note

Keywords

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