We cloned and expressed a full-length cDNA encoding a phospholipase D of type α (PLDα) from cabbage. Analysis of the cDNA predicted an 812-amino- acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.
|Number of pages||6|
|Journal||Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids|
|State||Published - 25 Mar 1999|
Bibliographical noteFunding Information:
This work was supported by the Ministry of Education, Republic of Korea (BSR1-96-3418), in part by the Korea Science and Engineering Foundation (KOSEF) through the Center for Molecular Catalysis at Seoul National University, and by the Ministry of Science and Technology of Korea (Grants KG1012, KG1091, KS1011, and KS1090).
- (Brassica oleracea var. capitata)
- cDNA sequence
- Phospholipase D