Molecular cloning and characterization of the mouse Peroxiredoxin V gene

Tae Hoon Lee, Sun Jung Kim, Sang Won Kang, Kyung Kwang Lee, Sue Goo Rhee, Dae Yeul Yu

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


We have cloned two cDNA isoforms as well as genomic sequences of the mouse Prx V gene and characterized their molecular genetic features. Two isoforms of the mouse Prx V cDNA were identified from liver and testis. The testis-originated long transcripts had extra 1164-bp 5'-UTR sequences compared to the liver-originated short transcripts. Primer extension and sequence analyses revealed that the two isoforms were presumably transcribed at the same gene locus. The gene was composed of six exons spanning 3.2 kb. The short transcript was abundantly expressed in the kidney, liver, and heart of the adult mouse tissues and in the extra-membrane of the 10.5 dpc embryos. The long transcript of 1985 bp was abundantly detected in testis with trace amounts in other tissues. Interestingly, in testis and fetus, only mRNA expression of the long form was identified. However, the protein expression was not found in testis, implying that the long form could not properly direct the protein expression. The long Prx V cDNA has eight uORFs in the extra 5'-UTR, which proceed the major ORF. The inability of protein expression for the long-form cDNA in testis suggests that the uORFs might inhibit translation of the major ORF and thereby confer the tissue-specific regulation of the mouse Prx V gene. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)356-362
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - 13 Apr 2000

Bibliographical note

Funding Information:
This work was supported by a grant (HS2490) from the Korea Ministry of Science and Technology.


  • Antioxidant enzyme
  • Bacterial artificial chromosome
  • Peroxiredoxin V
  • Upstream open reading frame


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