Modulation of sulfate renal transport by alterations in cell membrane fluidity

Hwa Jeong Lee; Sathyamangalam V. Balasubramanian; Heini Murer; Jurg Biber; Marilyn E. Morris

doi:10.1021/js990114c

Modulation of sulfate renal transport by alterations in cell membrane fluidity

Hwa Jeong Lee, Sathyamangalam V. Balasubramanian, Heini Murer, Jurg Biber, Marilyn E. Morris

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Changes in membrane fluidity have been shown to alter the sodium- dependent renal transport of glucose and phosphate; however, this has not been examined for sodium/sulfate cotransport in the renal proximal tubule. Sodium/sulfate cotransport regulates the homeostasis of sulfate in mammals. The objective of this study was to investigate the influence of alterations of membrane fluidity on sodium-coupled sulfate transport in the Madin-Darby canine kidney cells, which have been stably transfected with sodium/sulfate cotransporter (NaSi-1) cDNA (MDCK-Si). Preincubation of cells with 0.2 mM cholesterol significantly decreased the V(max) for sodium/sulfate cotransport (13.69 ± 1.11 vs 10.15 ± 1.17 nmol/mg protein/5 min, mean ± SD, n = 4, p< 0.01) with no significant alteration in K(m). The addition of benzyl alcohol (20 mM) to cells increased the V(max) of sulfate uptake by 20% (11.97 ± 0.91 vs 14.35 ± 0.56 nmol/mg protein/5 min, mean ±SD, n = 3, p < 0.05) with no significant change in K(m). Membrane fluidity, as measured by the fluorescence polarization of 1,6-diphenyl 1,3,5-hexatriene (DPH), was significantly increased in MDCK-Si cells treated with 20 mM benzyl alcohol and decreased in the cells preincubated with 0.2 mM cholesterol, compared with control cells. Our results suggest that alterations in membrane fluidity that may occur as a result of disease states, aging, and pregnancy may play an important role in the modulation of renal sodium/sulfate cotransport.

Original language	English
Pages (from-to)	976-980
Number of pages	5
Journal	Journal of Pharmaceutical Sciences
Volume	88
Issue number	10
DOIs	https://doi.org/10.1021/js990114c
State	Published - Oct 1999

Bibliographical note

Funding Information:
This work was supported by NSF grant IBN 9629470 and grants from the Western New York Kidney Foundation/Upstate Transplant Services and the Kapoor Charitable Foundation at SUNY at Buffalo (for M.E.M.). Additional support was provided by the Swiss National Science Foundation (for H.M.).

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@article{45cb228edf7d4e879022ceaa772cf3cb,

title = "Modulation of sulfate renal transport by alterations in cell membrane fluidity",

abstract = "Changes in membrane fluidity have been shown to alter the sodium- dependent renal transport of glucose and phosphate; however, this has not been examined for sodium/sulfate cotransport in the renal proximal tubule. Sodium/sulfate cotransport regulates the homeostasis of sulfate in mammals. The objective of this study was to investigate the influence of alterations of membrane fluidity on sodium-coupled sulfate transport in the Madin-Darby canine kidney cells, which have been stably transfected with sodium/sulfate cotransporter (NaSi-1) cDNA (MDCK-Si). Preincubation of cells with 0.2 mM cholesterol significantly decreased the V(max) for sodium/sulfate cotransport (13.69 ± 1.11 vs 10.15 ± 1.17 nmol/mg protein/5 min, mean ± SD, n = 4, p< 0.01) with no significant alteration in K(m). The addition of benzyl alcohol (20 mM) to cells increased the V(max) of sulfate uptake by 20% (11.97 ± 0.91 vs 14.35 ± 0.56 nmol/mg protein/5 min, mean ±SD, n = 3, p < 0.05) with no significant change in K(m). Membrane fluidity, as measured by the fluorescence polarization of 1,6-diphenyl 1,3,5-hexatriene (DPH), was significantly increased in MDCK-Si cells treated with 20 mM benzyl alcohol and decreased in the cells preincubated with 0.2 mM cholesterol, compared with control cells. Our results suggest that alterations in membrane fluidity that may occur as a result of disease states, aging, and pregnancy may play an important role in the modulation of renal sodium/sulfate cotransport.",

author = "Lee, {Hwa Jeong} and Balasubramanian, {Sathyamangalam V.} and Heini Murer and Jurg Biber and Morris, {Marilyn E.}",

note = "Funding Information: This work was supported by NSF grant IBN 9629470 and grants from the Western New York Kidney Foundation/Upstate Transplant Services and the Kapoor Charitable Foundation at SUNY at Buffalo (for M.E.M.). Additional support was provided by the Swiss National Science Foundation (for H.M.).",

year = "1999",

month = oct,

doi = "10.1021/js990114c",

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T1 - Modulation of sulfate renal transport by alterations in cell membrane fluidity

AU - Lee, Hwa Jeong

AU - Balasubramanian, Sathyamangalam V.

AU - Murer, Heini

AU - Biber, Jurg

AU - Morris, Marilyn E.

N1 - Funding Information: This work was supported by NSF grant IBN 9629470 and grants from the Western New York Kidney Foundation/Upstate Transplant Services and the Kapoor Charitable Foundation at SUNY at Buffalo (for M.E.M.). Additional support was provided by the Swiss National Science Foundation (for H.M.).

PY - 1999/10

Y1 - 1999/10

N2 - Changes in membrane fluidity have been shown to alter the sodium- dependent renal transport of glucose and phosphate; however, this has not been examined for sodium/sulfate cotransport in the renal proximal tubule. Sodium/sulfate cotransport regulates the homeostasis of sulfate in mammals. The objective of this study was to investigate the influence of alterations of membrane fluidity on sodium-coupled sulfate transport in the Madin-Darby canine kidney cells, which have been stably transfected with sodium/sulfate cotransporter (NaSi-1) cDNA (MDCK-Si). Preincubation of cells with 0.2 mM cholesterol significantly decreased the V(max) for sodium/sulfate cotransport (13.69 ± 1.11 vs 10.15 ± 1.17 nmol/mg protein/5 min, mean ± SD, n = 4, p< 0.01) with no significant alteration in K(m). The addition of benzyl alcohol (20 mM) to cells increased the V(max) of sulfate uptake by 20% (11.97 ± 0.91 vs 14.35 ± 0.56 nmol/mg protein/5 min, mean ±SD, n = 3, p < 0.05) with no significant change in K(m). Membrane fluidity, as measured by the fluorescence polarization of 1,6-diphenyl 1,3,5-hexatriene (DPH), was significantly increased in MDCK-Si cells treated with 20 mM benzyl alcohol and decreased in the cells preincubated with 0.2 mM cholesterol, compared with control cells. Our results suggest that alterations in membrane fluidity that may occur as a result of disease states, aging, and pregnancy may play an important role in the modulation of renal sodium/sulfate cotransport.

AB - Changes in membrane fluidity have been shown to alter the sodium- dependent renal transport of glucose and phosphate; however, this has not been examined for sodium/sulfate cotransport in the renal proximal tubule. Sodium/sulfate cotransport regulates the homeostasis of sulfate in mammals. The objective of this study was to investigate the influence of alterations of membrane fluidity on sodium-coupled sulfate transport in the Madin-Darby canine kidney cells, which have been stably transfected with sodium/sulfate cotransporter (NaSi-1) cDNA (MDCK-Si). Preincubation of cells with 0.2 mM cholesterol significantly decreased the V(max) for sodium/sulfate cotransport (13.69 ± 1.11 vs 10.15 ± 1.17 nmol/mg protein/5 min, mean ± SD, n = 4, p< 0.01) with no significant alteration in K(m). The addition of benzyl alcohol (20 mM) to cells increased the V(max) of sulfate uptake by 20% (11.97 ± 0.91 vs 14.35 ± 0.56 nmol/mg protein/5 min, mean ±SD, n = 3, p < 0.05) with no significant change in K(m). Membrane fluidity, as measured by the fluorescence polarization of 1,6-diphenyl 1,3,5-hexatriene (DPH), was significantly increased in MDCK-Si cells treated with 20 mM benzyl alcohol and decreased in the cells preincubated with 0.2 mM cholesterol, compared with control cells. Our results suggest that alterations in membrane fluidity that may occur as a result of disease states, aging, and pregnancy may play an important role in the modulation of renal sodium/sulfate cotransport.

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DO - 10.1021/js990114c

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SN - 0022-3549

VL - 88

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EP - 980

JO - Journal of Pharmaceutical Sciences

JF - Journal of Pharmaceutical Sciences

IS - 10

ER -

Modulation of sulfate renal transport by alterations in cell membrane fluidity

Abstract

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