Mobile Efficient Diagnostics of Infectious Diseases via On-Chip RT-qPCR: MEDIC-PCR

Kiran Shrestha; Seongryeong Kim; Jiyeon Han; Gabriela Morales Florez; Han Truong; Trung Hoang; Sajjan Parajuli; A. M. Tiara; Beomsoo Kim; Younsu Jung; Abdurhaman Teyib Abafogi; Yugyeong Lee; Seung Hyun Song; Jinkee Lee; Sungsu Park; Minhee Kang; Hee Jae Huh; Gyoujin Cho; Luke P. Lee

doi:10.1002/advs.202302072

Mobile Efficient Diagnostics of Infectious Diseases via On-Chip RT-qPCR: MEDIC-PCR

Kiran Shrestha, Seongryeong Kim, Jiyeon Han, Gabriela Morales Florez, Han Truong, Trung Hoang, Sajjan Parajuli, A. M. Tiara, Beomsoo Kim, Younsu Jung, Abdurhaman Teyib Abafogi, Yugyeong Lee, Seung Hyun Song, Jinkee Lee, Sungsu Park, Minhee Kang, Hee Jae Huh, Gyoujin Cho, Luke P. Lee

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The COVID-19 outbreak has caused public and global health crises. However, the lack of on-site fast, reliable, sensitive, and low-cost reverse transcription polymerase chain reaction (RT-PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors report a rapid mobile efficient diagnostics of infectious diseases via on-chip -RT-quantitative PCR (RT-qPCR): MEDIC-PCR. First, the authors use a roll-to-roll printing process to accomplish low-cost carbon-black-based disposable PCR chips that enable rapid LED-induced photothermal PCR cycles. The MEDIC-PCR can perform RT (3 min), and PCR (9 min) steps. Further, the cohort of 89 COVID-19 and 103 non-COVID-19 patients testing is completed by the MEDIC-PCR to show excellent diagnostic accuracy of 97%, sensitivity of 94%, and specificity of 98%. This MEDIC-PCR can contribute to the preventive global health in the face of a future pandemic.

Original language	English
Article number	2302072
Journal	Advanced Science
Volume	10
Issue number	28
DOIs	https://doi.org/10.1002/advs.202302072
State	Published - 6 Oct 2023

Bibliographical note

Publisher Copyright:
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.

Keywords

covid-19
diagnostics
low-cost
rapid
rt-qpcr

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10.1002/advs.202302072

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Shrestha, K., Kim, S., Han, J., Florez, G. M., Truong, H., Hoang, T., Parajuli, S., Tiara, A. M., Kim, B., Jung, Y., Abafogi, A. T., Lee, Y., Song, S. H., Lee, J., Park, S., Kang, M., Huh, H. J., Cho, G., & Lee, L. P. (2023). Mobile Efficient Diagnostics of Infectious Diseases via On-Chip RT-qPCR: MEDIC-PCR. Advanced Science, 10(28), Article 2302072. https://doi.org/10.1002/advs.202302072

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abstract = "The COVID-19 outbreak has caused public and global health crises. However, the lack of on-site fast, reliable, sensitive, and low-cost reverse transcription polymerase chain reaction (RT-PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors report a rapid mobile efficient diagnostics of infectious diseases via on-chip -RT-quantitative PCR (RT-qPCR): MEDIC-PCR. First, the authors use a roll-to-roll printing process to accomplish low-cost carbon-black-based disposable PCR chips that enable rapid LED-induced photothermal PCR cycles. The MEDIC-PCR can perform RT (3 min), and PCR (9 min) steps. Further, the cohort of 89 COVID-19 and 103 non-COVID-19 patients testing is completed by the MEDIC-PCR to show excellent diagnostic accuracy of 97%, sensitivity of 94%, and specificity of 98%. This MEDIC-PCR can contribute to the preventive global health in the face of a future pandemic.",

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author = "Kiran Shrestha and Seongryeong Kim and Jiyeon Han and Florez, {Gabriela Morales} and Han Truong and Trung Hoang and Sajjan Parajuli and Tiara, {A. M.} and Beomsoo Kim and Younsu Jung and Abafogi, {Abdurhaman Teyib} and Yugyeong Lee and Song, {Seung Hyun} and Jinkee Lee and Sungsu Park and Minhee Kang and Huh, {Hee Jae} and Gyoujin Cho and Lee, {Luke P.}",

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AU - Florez, Gabriela Morales

AU - Truong, Han

AU - Hoang, Trung

AU - Parajuli, Sajjan

AU - Tiara, A. M.

AU - Kim, Beomsoo

AU - Jung, Younsu

AU - Abafogi, Abdurhaman Teyib

AU - Lee, Yugyeong

AU - Song, Seung Hyun

AU - Lee, Jinkee

AU - Park, Sungsu

AU - Kang, Minhee

AU - Huh, Hee Jae

AU - Cho, Gyoujin

AU - Lee, Luke P.

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N2 - The COVID-19 outbreak has caused public and global health crises. However, the lack of on-site fast, reliable, sensitive, and low-cost reverse transcription polymerase chain reaction (RT-PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors report a rapid mobile efficient diagnostics of infectious diseases via on-chip -RT-quantitative PCR (RT-qPCR): MEDIC-PCR. First, the authors use a roll-to-roll printing process to accomplish low-cost carbon-black-based disposable PCR chips that enable rapid LED-induced photothermal PCR cycles. The MEDIC-PCR can perform RT (3 min), and PCR (9 min) steps. Further, the cohort of 89 COVID-19 and 103 non-COVID-19 patients testing is completed by the MEDIC-PCR to show excellent diagnostic accuracy of 97%, sensitivity of 94%, and specificity of 98%. This MEDIC-PCR can contribute to the preventive global health in the face of a future pandemic.

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Mobile Efficient Diagnostics of Infectious Diseases via On-Chip RT-qPCR: MEDIC-PCR

Abstract

Bibliographical note

Keywords

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