TY - JOUR
T1 - Mitochondria-Targeted Reaction-Based Fluorescent Probe for Hydrogen Sulfide
AU - Pak, Yen Leng
AU - Li, Jun
AU - Ko, Kyoung Chul
AU - Kim, Gyoungmi
AU - Lee, Jin Yong
AU - Yoon, Juyoung
N1 - Funding Information:
This research was supported by the grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (Grant No. 2012R1A3A2048814). Mass spectral data were obtained from Korea Basic Science Institute (Daegu) using a Jeol JMS 700 high-resolution mass spectrometer.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/5/17
Y1 - 2016/5/17
N2 - In this study, we developed a turn-on mitochondria-targeting hydrogen sulfide, "probe 1", based on the selective thiolysis of 7-nitro-1,2,3-benzoxadiazole amine moiety attached to the piperazine-based naphthalimide scaffold. Probe 1 exhibited excellent properties with 68-fold fluorescence enhancement, a low detection limit (2.46 μM), a low cytotoxicity, and a good selectivity toward hydrogen sulfide. The success of intracellular imaging indicated that probe 1 could be used in further applications for the investigation of biological functions and pathological roles of H2S in living systems.
AB - In this study, we developed a turn-on mitochondria-targeting hydrogen sulfide, "probe 1", based on the selective thiolysis of 7-nitro-1,2,3-benzoxadiazole amine moiety attached to the piperazine-based naphthalimide scaffold. Probe 1 exhibited excellent properties with 68-fold fluorescence enhancement, a low detection limit (2.46 μM), a low cytotoxicity, and a good selectivity toward hydrogen sulfide. The success of intracellular imaging indicated that probe 1 could be used in further applications for the investigation of biological functions and pathological roles of H2S in living systems.
UR - http://www.scopus.com/inward/record.url?scp=84969626849&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.6b00956
DO - 10.1021/acs.analchem.6b00956
M3 - Article
C2 - 27094621
AN - SCOPUS:84969626849
SN - 0003-2700
VL - 88
SP - 5476
EP - 5481
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 10
ER -