MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes

Heejin Lee; Sang Hoon Chun; Seo Yun Moon; Jung Sook Yoon; Hye Sung Won; Soon Auck Hong; Seo Ree Kim; Kwang Jae Cho; Keunsoo Kang; Sieun Lee; Young Ho Ahn; Ji Hyung Hong; Yoon Ho Ko

doi:10.3390/cancers14184373

MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes

Heejin Lee, Sang Hoon Chun, Seo Yun Moon, Jung Sook Yoon, Hye Sung Won, Soon Auck Hong, Seo Ree Kim, Kwang Jae Cho, Keunsoo Kang, Sieun Lee, Young Ho Ahn, Ji Hyung Hong, Yoon Ho Ko

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

miR-769-3p expression is suppressed in the stromal subtype of head and neck squamous cell carcinoma (HNSCC); however, its role in stromal HNSCC has not been fully elucidated. To investigate the biological relevance of miR-769-3p in the stromal phenotype, we established oral squamous cell cancer (OSCC) cell lines, namely CAL27, HSC3, and YD8, overexpressing miR-769-3p. miR-769-3p expression was positively and negatively correlated with interferon-gamma-related genes and MYC target gene sets, respectively. miR-769-3p decreased OSCC cell migration and invasion as well as mesenchymal marker expression and increased epithelial marker expression. Moreover, miR-769-3p enhanced OSCC cell sensitivity to 5-fluorouracil. High miR-769-3p expression was associated with good prognosis of HNSCC patients. Collectively, these results suggest that miR-769-3p suppression enhances stromal gene expression and promotes the epithelial-to-mesenchymal transition. Therefore, miR-769-3p may be a potential biomarker of the miRNA phenotype in OSCC patients.

Original language	English
Article number	4373
Journal	Cancers
Volume	14
Issue number	18
DOIs	https://doi.org/10.3390/cancers14184373
State	Published - Sep 2022

Bibliographical note

Funding Information:
This research was funded by the Catholic University of Korea Uijeongbu St. Mary’s Hospital Clinical Research Laboratory Foundation (grant number UJBCRL201840 to Y.H.K.); an industry–academic co-operation research fund from the Catholic University of Korea (grant number 5-2020-D0403-00001 to S.H.C.); Basic Science Research Program through the National Research Foundation funded by the Ministry of Education (grant number, NRF-2020R1I1A1A01066968 to H.L.).

Publisher Copyright:
© 2022 by the authors.

Keywords

EMT
head and neck neoplasm
miR-769
stroma
tumor-suppressive

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@article{625e25b6f8604b14aee9d709fc795cb9,

title = "MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes",

abstract = "miR-769-3p expression is suppressed in the stromal subtype of head and neck squamous cell carcinoma (HNSCC); however, its role in stromal HNSCC has not been fully elucidated. To investigate the biological relevance of miR-769-3p in the stromal phenotype, we established oral squamous cell cancer (OSCC) cell lines, namely CAL27, HSC3, and YD8, overexpressing miR-769-3p. miR-769-3p expression was positively and negatively correlated with interferon-gamma-related genes and MYC target gene sets, respectively. miR-769-3p decreased OSCC cell migration and invasion as well as mesenchymal marker expression and increased epithelial marker expression. Moreover, miR-769-3p enhanced OSCC cell sensitivity to 5-fluorouracil. High miR-769-3p expression was associated with good prognosis of HNSCC patients. Collectively, these results suggest that miR-769-3p suppression enhances stromal gene expression and promotes the epithelial-to-mesenchymal transition. Therefore, miR-769-3p may be a potential biomarker of the miRNA phenotype in OSCC patients.",

keywords = "EMT, head and neck neoplasm, miR-769, stroma, tumor-suppressive",

author = "Heejin Lee and Chun, {Sang Hoon} and Moon, {Seo Yun} and Yoon, {Jung Sook} and Won, {Hye Sung} and Hong, {Soon Auck} and Kim, {Seo Ree} and Cho, {Kwang Jae} and Keunsoo Kang and Sieun Lee and Ahn, {Young Ho} and Hong, {Ji Hyung} and Ko, {Yoon Ho}",

note = "Funding Information: This research was funded by the Catholic University of Korea Uijeongbu St. Mary{\textquoteright}s Hospital Clinical Research Laboratory Foundation (grant number UJBCRL201840 to Y.H.K.); an industry–academic co-operation research fund from the Catholic University of Korea (grant number 5-2020-D0403-00001 to S.H.C.); Basic Science Research Program through the National Research Foundation funded by the Ministry of Education (grant number, NRF-2020R1I1A1A01066968 to H.L.). Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = sep,

doi = "10.3390/cancers14184373",

language = "English",

volume = "14",

journal = "Cancers",

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AU - Lee, Heejin

AU - Chun, Sang Hoon

AU - Moon, Seo Yun

AU - Yoon, Jung Sook

AU - Won, Hye Sung

AU - Hong, Soon Auck

AU - Kim, Seo Ree

AU - Cho, Kwang Jae

AU - Kang, Keunsoo

AU - Lee, Sieun

AU - Ahn, Young Ho

AU - Hong, Ji Hyung

AU - Ko, Yoon Ho

N1 - Funding Information: This research was funded by the Catholic University of Korea Uijeongbu St. Mary’s Hospital Clinical Research Laboratory Foundation (grant number UJBCRL201840 to Y.H.K.); an industry–academic co-operation research fund from the Catholic University of Korea (grant number 5-2020-D0403-00001 to S.H.C.); Basic Science Research Program through the National Research Foundation funded by the Ministry of Education (grant number, NRF-2020R1I1A1A01066968 to H.L.). Publisher Copyright: © 2022 by the authors.

PY - 2022/9

Y1 - 2022/9

N2 - miR-769-3p expression is suppressed in the stromal subtype of head and neck squamous cell carcinoma (HNSCC); however, its role in stromal HNSCC has not been fully elucidated. To investigate the biological relevance of miR-769-3p in the stromal phenotype, we established oral squamous cell cancer (OSCC) cell lines, namely CAL27, HSC3, and YD8, overexpressing miR-769-3p. miR-769-3p expression was positively and negatively correlated with interferon-gamma-related genes and MYC target gene sets, respectively. miR-769-3p decreased OSCC cell migration and invasion as well as mesenchymal marker expression and increased epithelial marker expression. Moreover, miR-769-3p enhanced OSCC cell sensitivity to 5-fluorouracil. High miR-769-3p expression was associated with good prognosis of HNSCC patients. Collectively, these results suggest that miR-769-3p suppression enhances stromal gene expression and promotes the epithelial-to-mesenchymal transition. Therefore, miR-769-3p may be a potential biomarker of the miRNA phenotype in OSCC patients.

AB - miR-769-3p expression is suppressed in the stromal subtype of head and neck squamous cell carcinoma (HNSCC); however, its role in stromal HNSCC has not been fully elucidated. To investigate the biological relevance of miR-769-3p in the stromal phenotype, we established oral squamous cell cancer (OSCC) cell lines, namely CAL27, HSC3, and YD8, overexpressing miR-769-3p. miR-769-3p expression was positively and negatively correlated with interferon-gamma-related genes and MYC target gene sets, respectively. miR-769-3p decreased OSCC cell migration and invasion as well as mesenchymal marker expression and increased epithelial marker expression. Moreover, miR-769-3p enhanced OSCC cell sensitivity to 5-fluorouracil. High miR-769-3p expression was associated with good prognosis of HNSCC patients. Collectively, these results suggest that miR-769-3p suppression enhances stromal gene expression and promotes the epithelial-to-mesenchymal transition. Therefore, miR-769-3p may be a potential biomarker of the miRNA phenotype in OSCC patients.

KW - EMT

KW - head and neck neoplasm

KW - miR-769

KW - stroma

KW - tumor-suppressive

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DO - 10.3390/cancers14184373

M3 - Article

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JO - Cancers

JF - Cancers

IS - 18

M1 - 4373

ER -

MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes

Abstract

Bibliographical note

Keywords

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