MicroRNA-769-3p Acts as a Prognostic Factor in Oral Squamous Cell Cancer by Modulating Stromal Genes

Heejin Lee, Sang Hoon Chun, Seo Yun Moon, Jung Sook Yoon, Hye Sung Won, Soon Auck Hong, Seo Ree Kim, Kwang Jae Cho, Keunsoo Kang, Sieun Lee, Young Ho Ahn, Ji Hyung Hong, Yoon Ho Ko

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


miR-769-3p expression is suppressed in the stromal subtype of head and neck squamous cell carcinoma (HNSCC); however, its role in stromal HNSCC has not been fully elucidated. To investigate the biological relevance of miR-769-3p in the stromal phenotype, we established oral squamous cell cancer (OSCC) cell lines, namely CAL27, HSC3, and YD8, overexpressing miR-769-3p. miR-769-3p expression was positively and negatively correlated with interferon-gamma-related genes and MYC target gene sets, respectively. miR-769-3p decreased OSCC cell migration and invasion as well as mesenchymal marker expression and increased epithelial marker expression. Moreover, miR-769-3p enhanced OSCC cell sensitivity to 5-fluorouracil. High miR-769-3p expression was associated with good prognosis of HNSCC patients. Collectively, these results suggest that miR-769-3p suppression enhances stromal gene expression and promotes the epithelial-to-mesenchymal transition. Therefore, miR-769-3p may be a potential biomarker of the miRNA phenotype in OSCC patients.

Original languageEnglish
Article number4373
Issue number18
StatePublished - Sep 2022

Bibliographical note

Funding Information:
This research was funded by the Catholic University of Korea Uijeongbu St. Mary’s Hospital Clinical Research Laboratory Foundation (grant number UJBCRL201840 to Y.H.K.); an industry–academic co-operation research fund from the Catholic University of Korea (grant number 5-2020-D0403-00001 to S.H.C.); Basic Science Research Program through the National Research Foundation funded by the Ministry of Education (grant number, NRF-2020R1I1A1A01066968 to H.L.).

Publisher Copyright:
© 2022 by the authors.


  • EMT
  • head and neck neoplasm
  • miR-769
  • stroma
  • tumor-suppressive


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