Micropatterning of bacteria on two-dimensional lattice protein surface observed by atomic force microscopy

Y. J. Oh, W. Jo, J. Lim, S. Park, Y. S. Kim, Y. Kim

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In this study, we characterized the two-dimensional lattice of bovine serum albumin (BSA) as a chemical and physical barrier against bacterial adhesion, using fluorescence microscopy and atomic force microscopy (AFM). The lattice of BSA on glass surface was fabricated by micro-contact printing (μCP), which is a useful way to pattern a wide range of molecules into microscale features on different types of substrates. The contact-mode AFM measurements showed that the average height of the printed BSA monolayer was 5-6 nm. Escherichia coli adhered rapidly on bare glass slide, while the bacterial adhesion was minimized on the lattices in the range of 1-3 μm2. Especially, the bacterial adhesion was completely inhibited on a 1 μm2 lattice. The results suggest that the anti-adhesion effects are due by the steric repulsion forces exerted by BSA.

Original languageEnglish
Pages (from-to)1124-1127
Number of pages4
JournalUltramicroscopy
Volume108
Issue number10
DOIs
StatePublished - Sep 2008

Keywords

  • AFM
  • Patterning
  • Protein

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