Abstract
In this study, we characterized the two-dimensional lattice of bovine serum albumin (BSA) as a chemical and physical barrier against bacterial adhesion, using fluorescence microscopy and atomic force microscopy (AFM). The lattice of BSA on glass surface was fabricated by micro-contact printing (μCP), which is a useful way to pattern a wide range of molecules into microscale features on different types of substrates. The contact-mode AFM measurements showed that the average height of the printed BSA monolayer was 5-6 nm. Escherichia coli adhered rapidly on bare glass slide, while the bacterial adhesion was minimized on the lattices in the range of 1-3 μm2. Especially, the bacterial adhesion was completely inhibited on a 1 μm2 lattice. The results suggest that the anti-adhesion effects are due by the steric repulsion forces exerted by BSA.
Original language | English |
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Pages (from-to) | 1124-1127 |
Number of pages | 4 |
Journal | Ultramicroscopy |
Volume | 108 |
Issue number | 10 |
DOIs | |
State | Published - Sep 2008 |
Keywords
- AFM
- Patterning
- Protein