Abstract
We applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 665-667 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| State | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 |
Publication series
| Name | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country/Territory | Korea, Republic of |
| City | Gyeongju |
| Period | 25/10/15 → 29/10/15 |
Bibliographical note
Publisher Copyright:© 15CBMS-0001.
Keywords
- Microcontact printing
- Neural stem cell
- Neuron