Microfluidic ping pong for cytokine detection

R. D. Sochol; L. Lo; R. Ruelos; V. Chang; D. Bahri; K. Iwai; J. C. Lo; M. Dueck; L. P. Lee; L. Lin

doi:10.1109/MEMSYS.2011.5734580

Microfluidic ping pong for cytokine detection

R. D. Sochol
, L. Lo
, R. Ruelos
, V. Chang
, D. Bahri
, K. Iwai
, J. C. Lo
, M. Dueck
, L. P. Lee
, L. Lin

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Scopus citations

Abstract

Dynamic bead-based microarrays offer an ideal platform for chemical and biological applications, including on-site chemical analysis, point-of-care medical diagnostics and synthetic biology on a chip. Despite significant development of dynamic microarrays, challenges have remained for achieving multi-stage fluidic reactions and analyses. Here we present a novel bead-based microarray platform - Microfluidic Ping Pong (MPP) - and demonstrate this system by detecting the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-step aptamer-based sandwich assay with five additional wash steps. Using aptamer beacons immobilized on 15 μm diameter polystyrene microbeads, 100 pM concentrations of IFN-γ were detected in the MPP system.

Original language	English
Title of host publication	2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
Pages	936-939
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2011.5734580
State	Published - 2011
Event	24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico Duration: 23 Jan 2011 → 27 Jan 2011

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
Country/Territory	Mexico
City	Cancun
Period	23/01/11 → 27/01/11

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10.1109/MEMSYS.2011.5734580

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Sochol, R. D., Lo, L., Ruelos, R., Chang, V., Bahri, D., Iwai, K., Lo, J. C., Dueck, M., Lee, L. P., & Lin, L. (2011). Microfluidic ping pong for cytokine detection. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011 (pp. 936-939). Article 5734580 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734580

@inproceedings{cbf2337436c4425fa1f025bc1c0bd916,

title = "Microfluidic ping pong for cytokine detection",

abstract = "Dynamic bead-based microarrays offer an ideal platform for chemical and biological applications, including on-site chemical analysis, point-of-care medical diagnostics and synthetic biology on a chip. Despite significant development of dynamic microarrays, challenges have remained for achieving multi-stage fluidic reactions and analyses. Here we present a novel bead-based microarray platform - Microfluidic Ping Pong (MPP) - and demonstrate this system by detecting the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-step aptamer-based sandwich assay with five additional wash steps. Using aptamer beacons immobilized on 15 μm diameter polystyrene microbeads, 100 pM concentrations of IFN-γ were detected in the MPP system.",

author = "Sochol, \{R. D.\} and L. Lo and R. Ruelos and V. Chang and D. Bahri and K. Iwai and Lo, \{J. C.\} and M. Dueck and Lee, \{L. P.\} and L. Lin",

year = "2011",

doi = "10.1109/MEMSYS.2011.5734580",

language = "English",

isbn = "9781424496327",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "936--939",

booktitle = "2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011",

note = "24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 ; Conference date: 23-01-2011 Through 27-01-2011",

}

Sochol, RD, Lo, L, Ruelos, R, Chang, V, Bahri, D, Iwai, K, Lo, JC, Dueck, M, Lee, LP & Lin, L 2011, Microfluidic ping pong for cytokine detection. in 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011., 5734580, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 936-939, 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011, Cancun, Mexico, 23/01/11. https://doi.org/10.1109/MEMSYS.2011.5734580

Microfluidic ping pong for cytokine detection. / Sochol, R. D.; Lo, L.; Ruelos, R. et al.
2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 936-939 5734580 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Microfluidic ping pong for cytokine detection

AU - Sochol, R. D.

AU - Lo, L.

AU - Ruelos, R.

AU - Chang, V.

AU - Bahri, D.

AU - Iwai, K.

AU - Lo, J. C.

AU - Dueck, M.

AU - Lee, L. P.

AU - Lin, L.

PY - 2011

Y1 - 2011

N2 - Dynamic bead-based microarrays offer an ideal platform for chemical and biological applications, including on-site chemical analysis, point-of-care medical diagnostics and synthetic biology on a chip. Despite significant development of dynamic microarrays, challenges have remained for achieving multi-stage fluidic reactions and analyses. Here we present a novel bead-based microarray platform - Microfluidic Ping Pong (MPP) - and demonstrate this system by detecting the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-step aptamer-based sandwich assay with five additional wash steps. Using aptamer beacons immobilized on 15 μm diameter polystyrene microbeads, 100 pM concentrations of IFN-γ were detected in the MPP system.

AB - Dynamic bead-based microarrays offer an ideal platform for chemical and biological applications, including on-site chemical analysis, point-of-care medical diagnostics and synthetic biology on a chip. Despite significant development of dynamic microarrays, challenges have remained for achieving multi-stage fluidic reactions and analyses. Here we present a novel bead-based microarray platform - Microfluidic Ping Pong (MPP) - and demonstrate this system by detecting the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-step aptamer-based sandwich assay with five additional wash steps. Using aptamer beacons immobilized on 15 μm diameter polystyrene microbeads, 100 pM concentrations of IFN-γ were detected in the MPP system.

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DO - 10.1109/MEMSYS.2011.5734580

M3 - Conference contribution

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SN - 9781424496327

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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BT - 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011

T2 - 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011

Y2 - 23 January 2011 through 27 January 2011

ER -

Microfluidic ping pong for cytokine detection

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