TY - GEN
T1 - Microfluidic ping pong for cytokine detection
AU - Sochol, R. D.
AU - Lo, L.
AU - Ruelos, R.
AU - Chang, V.
AU - Bahri, D.
AU - Iwai, K.
AU - Lo, J. C.
AU - Dueck, M.
AU - Lee, L. P.
AU - Lin, L.
PY - 2011
Y1 - 2011
N2 - Dynamic bead-based microarrays offer an ideal platform for chemical and biological applications, including on-site chemical analysis, point-of-care medical diagnostics and synthetic biology on a chip. Despite significant development of dynamic microarrays, challenges have remained for achieving multi-stage fluidic reactions and analyses. Here we present a novel bead-based microarray platform - Microfluidic Ping Pong (MPP) - and demonstrate this system by detecting the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-step aptamer-based sandwich assay with five additional wash steps. Using aptamer beacons immobilized on 15 μm diameter polystyrene microbeads, 100 pM concentrations of IFN-γ were detected in the MPP system.
AB - Dynamic bead-based microarrays offer an ideal platform for chemical and biological applications, including on-site chemical analysis, point-of-care medical diagnostics and synthetic biology on a chip. Despite significant development of dynamic microarrays, challenges have remained for achieving multi-stage fluidic reactions and analyses. Here we present a novel bead-based microarray platform - Microfluidic Ping Pong (MPP) - and demonstrate this system by detecting the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-step aptamer-based sandwich assay with five additional wash steps. Using aptamer beacons immobilized on 15 μm diameter polystyrene microbeads, 100 pM concentrations of IFN-γ were detected in the MPP system.
UR - http://www.scopus.com/inward/record.url?scp=79953796138&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2011.5734580
DO - 10.1109/MEMSYS.2011.5734580
M3 - Conference contribution
AN - SCOPUS:79953796138
SN - 9781424496327
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 936
EP - 939
BT - 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
T2 - 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
Y2 - 23 January 2011 through 27 January 2011
ER -