Microfluidic ping pong for cytokine detection

R. D. Sochol, L. Lo, R. Ruelos, V. Chang, D. Bahri, K. Iwai, J. C. Lo, M. Dueck, L. P. Lee, L. Lin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

Dynamic bead-based microarrays offer an ideal platform for chemical and biological applications, including on-site chemical analysis, point-of-care medical diagnostics and synthetic biology on a chip. Despite significant development of dynamic microarrays, challenges have remained for achieving multi-stage fluidic reactions and analyses. Here we present a novel bead-based microarray platform - Microfluidic Ping Pong (MPP) - and demonstrate this system by detecting the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-step aptamer-based sandwich assay with five additional wash steps. Using aptamer beacons immobilized on 15 μm diameter polystyrene microbeads, 100 pM concentrations of IFN-γ were detected in the MPP system.

Original languageEnglish
Title of host publication2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
Pages936-939
Number of pages4
DOIs
StatePublished - 2011
Event24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico
Duration: 23 Jan 201127 Jan 2011

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

Conference24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
Country/TerritoryMexico
CityCancun
Period23/01/1127/01/11

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