Microfluidic environment for high density hepatocyte culture

Mimi Y. Zhang; Philip J. Lee; Paul J. Hung; Terry Johnson; Luke P. Lee; Mohammed R.K. Mofrad

doi:10.1007/s10544-007-9116-9

Microfluidic environment for high density hepatocyte culture

Mimi Y. Zhang, Philip J. Lee, Paul J. Hung, Terry Johnson, Luke P. Lee, Mohammed R.K. Mofrad

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

We present a microfluidic bioreactor for culturing high-density arrays of hepatocytes in a tissue-like micro-architecture. The microfluidic environment mimicked physiological liver mass transport, enabling sustained culture of high density cells (>2,000 cells/mm²) without nutrient limitation for over 1 week. The key feature of this design was a microporous microfluidic barrier that formed a sieved-pocket to concentrate cells during loading. Nutrient depletion within the cell mass was avoided by maintaining a continuous flow of medium (10 μl/day) that diffused across the porous barrier. Human hepatoma cells (HepG2/C3A) remained viable and functional as demonstrated by fluorescent viability assays and secretion of albumin for the one-week culture period.

Original language	English
Pages (from-to)	117-121
Number of pages	5
Journal	Biomedical Microdevices
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/s10544-007-9116-9
State	Published - Feb 2008

Bibliographical note

Funding Information:
work was funded by the NSF SBIR

Keywords

Bioreactor
Cell culture
Hepatocytes
Microfluidics

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10.1007/s10544-007-9116-9

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title = "Microfluidic environment for high density hepatocyte culture",

abstract = "We present a microfluidic bioreactor for culturing high-density arrays of hepatocytes in a tissue-like micro-architecture. The microfluidic environment mimicked physiological liver mass transport, enabling sustained culture of high density cells (>2,000 cells/mm2) without nutrient limitation for over 1 week. The key feature of this design was a microporous microfluidic barrier that formed a sieved-pocket to concentrate cells during loading. Nutrient depletion within the cell mass was avoided by maintaining a continuous flow of medium (10 μl/day) that diffused across the porous barrier. Human hepatoma cells (HepG2/C3A) remained viable and functional as demonstrated by fluorescent viability assays and secretion of albumin for the one-week culture period.",

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AU - Zhang, Mimi Y.

AU - Lee, Philip J.

AU - Hung, Paul J.

AU - Johnson, Terry

AU - Lee, Luke P.

AU - Mofrad, Mohammed R.K.

N1 - Funding Information: work was funded by the NSF SBIR

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AB - We present a microfluidic bioreactor for culturing high-density arrays of hepatocytes in a tissue-like micro-architecture. The microfluidic environment mimicked physiological liver mass transport, enabling sustained culture of high density cells (>2,000 cells/mm2) without nutrient limitation for over 1 week. The key feature of this design was a microporous microfluidic barrier that formed a sieved-pocket to concentrate cells during loading. Nutrient depletion within the cell mass was avoided by maintaining a continuous flow of medium (10 μl/day) that diffused across the porous barrier. Human hepatoma cells (HepG2/C3A) remained viable and functional as demonstrated by fluorescent viability assays and secretion of albumin for the one-week culture period.

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Microfluidic environment for high density hepatocyte culture

Abstract

Bibliographical note

Keywords

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