Microfluidic cell cutlure array for quantitative biology

Philip J. Lee; Paul J. Hung; Luke P. Lee

Microfluidic cell cutlure array for quantitative biology

Philip J. Lee
, Paul J. Hung
, Luke P. Lee

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

A nanoliter scale microbioreactor array was designed for multiplexed quantitative cell biology. An addressable 8x8 array of 3 nanoliter chambers was demonstrated for observing the serum response of HeLa human cancer cells in 64 parallel cultures over 5 days. The microbioreactor array provided a continuous flow culture environment with a Peclet number (0.02) and shear stress (0.01 Pa) that approximated in vivo tissue conditions without limiting mass transport (10 s nutrient turnover). This microfluidic design overcomes the major problems encountered in multiplexing nanoliter culture environments by enabling uniform cell loading and eliminating shear and pressure stresses on cultured cells.

Original language	English
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Transducer Research Foundation
Pages	1413-1415
Number of pages	3
ISBN (Print)	0974361119, 9780974361116
State	Published - 2005
Event	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States Duration: 9 Oct 2005 → 13 Oct 2005

Publication series

Name	Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume	1

Conference

Conference	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/Territory	United States
City	Boston, MA
Period	9/10/05 → 13/10/05

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell biology
Culture array
Microfluidic
Systems biology

Cite this

Lee, P. J., Hung, P. J., & Lee, L. P. (2005). Microfluidic cell cutlure array for quantitative biology. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1413-1415). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.

Lee, Philip J. ; Hung, Paul J. ; Lee, Luke P. / Microfluidic cell cutlure array for quantitative biology. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. pp. 1413-1415 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

@inproceedings{cbca71173f794e219df7f257cc32fad2,

title = "Microfluidic cell cutlure array for quantitative biology",

abstract = "A nanoliter scale microbioreactor array was designed for multiplexed quantitative cell biology. An addressable 8x8 array of 3 nanoliter chambers was demonstrated for observing the serum response of HeLa human cancer cells in 64 parallel cultures over 5 days. The microbioreactor array provided a continuous flow culture environment with a Peclet number (0.02) and shear stress (0.01 Pa) that approximated in vivo tissue conditions without limiting mass transport (10 s nutrient turnover). This microfluidic design overcomes the major problems encountered in multiplexing nanoliter culture environments by enabling uniform cell loading and eliminating shear and pressure stresses on cultured cells.",

keywords = "Cell biology, Culture array, Microfluidic, Systems biology",

author = "Lee, \{Philip J.\} and Hung, \{Paul J.\} and Lee, \{Luke P.\}",

year = "2005",

language = "English",

isbn = "0974361119",

series = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Transducer Research Foundation",

pages = "1413--1415",

booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference",

note = "9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 ; Conference date: 09-10-2005 Through 13-10-2005",

}

Lee, PJ, Hung, PJ & Lee, LP 2005, Microfluidic cell cutlure array for quantitative biology. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, vol. 1, Transducer Research Foundation, pp. 1413-1415, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 9/10/05.

Microfluidic cell cutlure array for quantitative biology. / Lee, Philip J.; Hung, Paul J.; Lee, Luke P.
Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. p. 1413-1415 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Microfluidic cell cutlure array for quantitative biology

AU - Lee, Philip J.

AU - Hung, Paul J.

AU - Lee, Luke P.

PY - 2005

Y1 - 2005

N2 - A nanoliter scale microbioreactor array was designed for multiplexed quantitative cell biology. An addressable 8x8 array of 3 nanoliter chambers was demonstrated for observing the serum response of HeLa human cancer cells in 64 parallel cultures over 5 days. The microbioreactor array provided a continuous flow culture environment with a Peclet number (0.02) and shear stress (0.01 Pa) that approximated in vivo tissue conditions without limiting mass transport (10 s nutrient turnover). This microfluidic design overcomes the major problems encountered in multiplexing nanoliter culture environments by enabling uniform cell loading and eliminating shear and pressure stresses on cultured cells.

AB - A nanoliter scale microbioreactor array was designed for multiplexed quantitative cell biology. An addressable 8x8 array of 3 nanoliter chambers was demonstrated for observing the serum response of HeLa human cancer cells in 64 parallel cultures over 5 days. The microbioreactor array provided a continuous flow culture environment with a Peclet number (0.02) and shear stress (0.01 Pa) that approximated in vivo tissue conditions without limiting mass transport (10 s nutrient turnover). This microfluidic design overcomes the major problems encountered in multiplexing nanoliter culture environments by enabling uniform cell loading and eliminating shear and pressure stresses on cultured cells.

KW - Cell biology

KW - Culture array

KW - Microfluidic

KW - Systems biology

UR - https://www.scopus.com/pages/publications/84902519319

M3 - Conference contribution

AN - SCOPUS:84902519319

SN - 0974361119

SN - 9780974361116

T3 - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 1413

EP - 1415

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference

PB - Transducer Research Foundation

T2 - 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005

Y2 - 9 October 2005 through 13 October 2005

ER -

Lee PJ, Hung PJ, Lee LP. Microfluidic cell cutlure array for quantitative biology. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation. 2005. p. 1413-1415. (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Microfluidic cell cutlure array for quantitative biology

Abstract

Publication series

Conference

UN SDGs

Keywords

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Cite this