@inproceedings{cbca71173f794e219df7f257cc32fad2,
title = "Microfluidic cell cutlure array for quantitative biology",
abstract = "A nanoliter scale microbioreactor array was designed for multiplexed quantitative cell biology. An addressable 8x8 array of 3 nanoliter chambers was demonstrated for observing the serum response of HeLa human cancer cells in 64 parallel cultures over 5 days. The microbioreactor array provided a continuous flow culture environment with a Peclet number (0.02) and shear stress (0.01 Pa) that approximated in vivo tissue conditions without limiting mass transport (10 s nutrient turnover). This microfluidic design overcomes the major problems encountered in multiplexing nanoliter culture environments by enabling uniform cell loading and eliminating shear and pressure stresses on cultured cells.",
keywords = "Cell biology, Culture array, Microfluidic, Systems biology",
author = "Lee, {Philip J.} and Hung, {Paul J.} and Lee, {Luke P.}",
year = "2005",
language = "English",
isbn = "0974361119",
series = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Transducer Research Foundation",
pages = "1413--1415",
booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference",
note = "9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 ; Conference date: 09-10-2005 Through 13-10-2005",
}
