TY - GEN
T1 - Microfluidic cell culture array for on-chip cell biology
AU - Lee, P. J.
AU - Hung, P. J.
AU - Lee, L. P.
PY - 2005
Y1 - 2005
N2 - An arrayed microfluidic platform was developed for cell biology applications. Using soft lithography methods, individual cell culture units were fabricated with a diameter from 50-500 μm. The channel height aspect ratio modulation (CHARM) design allowed fine control of fluidic flow through the array, enabling uniform cell loading, reduced shear stress, and decoupling of fluid transport from cell growth. A variety of adherent mammalian tissue culture cell lines were successfully cultured in the array with continuous perfusion of medium for over 2 weeks. Individual culture units were multiplexed in 2 dimensions to create 6×6 and 8×8 arrays, allowing row and column addressing to provide a unique culture environment in each microchamber. Demonstrated functionalities for cell biology research included repeated cell passaging in a fully enclosed microenvironment, fluorescent cell analysis (live and fixed), on-chip protein tranfection, and multiplexed long-term "flow-cell" microscopy.
AB - An arrayed microfluidic platform was developed for cell biology applications. Using soft lithography methods, individual cell culture units were fabricated with a diameter from 50-500 μm. The channel height aspect ratio modulation (CHARM) design allowed fine control of fluidic flow through the array, enabling uniform cell loading, reduced shear stress, and decoupling of fluid transport from cell growth. A variety of adherent mammalian tissue culture cell lines were successfully cultured in the array with continuous perfusion of medium for over 2 weeks. Individual culture units were multiplexed in 2 dimensions to create 6×6 and 8×8 arrays, allowing row and column addressing to provide a unique culture environment in each microchamber. Demonstrated functionalities for cell biology research included repeated cell passaging in a fully enclosed microenvironment, fluorescent cell analysis (live and fixed), on-chip protein tranfection, and multiplexed long-term "flow-cell" microscopy.
UR - http://www.scopus.com/inward/record.url?scp=33845286205&partnerID=8YFLogxK
U2 - 10.1109/MMB.2005.1548481
DO - 10.1109/MMB.2005.1548481
M3 - Conference contribution
AN - SCOPUS:33845286205
SN - 0780387112
SN - 9780780387119
T3 - 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
SP - 382
EP - 384
BT - 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
T2 - 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Y2 - 12 May 2005 through 15 May 2005
ER -