Abstract
We present an integrated microfluidic platform developed for automated cell culture and lysate analysis. Through the combination of previous technologies developed in our lab - on-chip cell culture and electrochemical lysis - we have designed, fabricated and characterized a platform that cultures cells for extended periods and lyses them on demand without the need for the addition of lysing or washing reagents. We have accomplished the successful culture and lysis of four cell lines: HeLa, MCF7, Jurkat, and CHO. Cells were cultured successfully for greater than five days, and complete on-demand lysis was demonstrated by local electrochemical generation of hydroxide. Lysate was shown to contain both nucleic acids and proteins. Additionally, the biological activity of the lysate was investigated by studying the effects of electrochemical lysis on the enzymatic activity of a model enzyme.
| Original language | English |
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| Title of host publication | Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
| Editors | Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 736-738 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806407 |
| State | Published - 2007 |
| Event | 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France Duration: 7 Oct 2007 → 11 Oct 2007 |
Publication series
| Name | Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
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Conference
| Conference | 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
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| Country/Territory | France |
| City | Paris |
| Period | 7/10/07 → 11/10/07 |
Bibliographical note
Publisher Copyright:© 2007 CBMS.
Keywords
- Cell-based bioassay
- Electrochemical cell lysis
- Microfluidic cell culture