Microfluidic bead-based sensing platform for monitoring kinase activity

Seung Hwan Lee, Hyun Woo Rhee, Danny van Noort, Hong Jai Lee, Hee Ho Park, Ik Soo Shin, Jong In Hong, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Protein kinases control cellular functions by regulating protein phosphorylation. Monitoring protein kinase activity is essential for medical diagnosis and drug screening. Here, we present a novel microfluidic device for performing simple and versatile protein kinase assays, which utilizes a microbead-based chemosensor. An automatic mix-and-measure technique was achieved using integrated pneumatic valves. After mixing each reagent for the kinase assay, the mixture was transferred to the sensing chamber. Then, phosphorylated and fluorescence-labeled peptides were captured and detected by the chemosensor. A fluorescence signal was observed depending on the presence of the kinase. Furthermore, activities of various kinases in the cell lysate and the inhibitory effect of specific chemicals on the kinases were monitored. These results indicate that chemosensor-based microfluidic chips can be developed as a versatile kinase assay system.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalBiosensors and Bioelectronics
StatePublished - 15 Jul 2014

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Education, Science, and Technology (MEST) (Nos. 2013069511 , 2013005843 , and 2013003890 ). D. van Noort was supported by Brain Pool 2012 through KOFST in Korea.


  • Chemosensor
  • Kinase assay
  • Microfluidic device
  • Protein kinase activity


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