Microbial synthesis of plant oxylipins from γ-linolenic acid through designed biotransformation pathways

Sae Um Kim, Kyoung Rok Kim, Ji Won Kim, Soomin Kim, Yong Uk Kwon, Deok Kun Oh, Jin Byung Park

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Secondary metabolites of plants are often difficult to synthesize in high yields because of the large complexity of the biosynthetic pathways and challenges encountered in the functional expression of the required biosynthetic enzymes in microbial cells. In this study, the biosynthesis of plant oxylipins - a family of oxygenated unsaturated carboxylic acids - was explored to enable a high-yield production through a designed microbial synthetic system harboring a set of microbial enzymes (i.e., fatty acid double-bond hydratases, alcohol dehydrogenases, Baeyer-Villiger monooxygenases, and esterases) to produce a variety of unsaturated carboxylic acids from γ-linolenic acid. The whole cell system of the recombinant Escherichia coli efficiently produced (6Z,9Z)-12-hydroxydodeca-6,9-dienoic acid (7), (Z)-9-hydroxynon-6-enoic acid (15), (Z)-dec-4-enedioic acid (17), and (6Z,9Z)-13-hydroxyoctadeca-6,9-dienoic acid (2). This study demonstrated that various secondary metabolites of plants can be produced by implementing artificial biosynthetic pathways into whole-cell biocatalysis.

Original languageEnglish
Pages (from-to)2773-2781
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number10
DOIs
StatePublished - 18 Mar 2015

Keywords

  • biotransformation
  • Escherichia coli
  • plant oxylipins
  • whole-cell biocatalysis
  • γ-linolenic acid

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