Microbial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains

Si Hong Park, Sun Ae Kim, Peter M. Rubinelli, Stephanie M. Roto, Steven C. Ricke

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Previously, we constructed and characterized the vaccine efficacy of Salmonella Typhimurium mutant strains in poultry with either inducible mviN expression (PBAD-mviN) or methionine auxotrophy (ΔΔmetRmetD). The aim of the present study was to assess potential impact of these Salmonella vaccine strains on the cecal microbiota using a next generation sequencing (NGS). The cecal microbial community obtained from unvaccinated (group 1) and vaccinated chickens (group 2, vaccinated with PBAD-mviN; group 3, vaccinated with wild type; group 4, vaccinated with ΔΔmetRmetD) were subjected to microbiome sequencing analysis with an Illumina MiSeq platform. The NGS microbiome analysis of chicken ceca revealed considerable changes in microbial composition in the presence of the different vaccine strains and exhibited detectable patterns of distinctive clustering among the respective groups (the R value of unweighted PCoA plot was 0.68). The present study indicates that different S. Typhimurium vaccine strains can differentially influence the microbiota of the ceca in terms of presence but not in the relative abundance of microbiota.

Original languageEnglish
Pages (from-to)3204-3208
Number of pages5
JournalVaccine
Volume35
Issue number24
DOIs
StatePublished - 31 May 2017

Keywords

  • Microbiome
  • Poultry
  • Salmonella
  • Sequencing
  • Vaccine

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