Microbial community analysis of perchlorate-reducing cultures growing on zero-valent iron

Ahjeong Son, Carl J. Schmidt, Hyejin Shin, Daniel K. Cha

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Anaerobic microbial mixed cultures demonstrated its ability to completely remove perchlorate in the presence of zero-valent iron. In order to understand the major microbial reaction in the iron-supported culture, community analysis comprising of microbial fatty acids and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) techniques was performed for perchlorate reducing cultures. Analysis of fatty acid methyl esters (FAMEs) and subsequent principal component analysis (PCA) showed clear distinctions not only between iron-supported perchlorate reducing culture and seed bacteria, but also among perchlorate-reducing cultures receiving different electron donors. The DGGE pattern targeting the chlorite dismutase (cld) gene showed that iron-supported perchlorate reducing culture is similar to hydrogen-fed cultures as compared to acetate-fed culture. The phylogenetic tree suggested that the dominant microbial reaction may be a combination of the autotrophic and heterotrophic reduction of perchlorate. Both molecular and chemotaxonomic experimental results support further understanding in the function of zero-valent iron as an adequate electron source for enhancing the microbial perchlorate reduction in natural and engineered systems.

Original languageEnglish
Pages (from-to)669-676
Number of pages8
JournalJournal of Hazardous Materials
Issue number2-3
StatePublished - 30 Jan 2011


  • Chlorite dismutase (cld) gene
  • FAME
  • Perchlorate
  • Phylogenetic analysis
  • Zero-valent iron


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