TY - JOUR
T1 - Micro corona discharge based cell lysis method suitable for inhibitor resistant bacterial sensing systems
AU - Lee, Eun Hee
AU - Chua, Beelee
AU - Son, Ahjeong
N1 - Funding Information:
This project was made possible by funding provided by National Science Foundation (CAREER award #1054768 ), National Research Foundation of Korea ( #2014003129 ), and Ministry of Environment in Korea (GAIA, G114-00056-04031 ).
Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - We demonstrated a bacterial cell lysis method suitable for inhibitor resistant bacterial detection systems where purification of extracted DNA is not necessary. The presented method potentially improves the field portability of such systems. It allows cell lysis and DNA extraction to be performed without the use of bead mill, sonication, thermal cycling, additional reagents or enzymes. Bacterial cell lysis is achieved in a single step by pumping ozone generated by a micro corona discharge into the bacterial sample. The results with Pseudomonas putida as the target bacteria showed that it was capable of achieving 98.5 ± 0.2% lysis (normalized to 1 min of sonication at 10 W) after 10 min of treatment at a flow rate of 38 ml/min and an applied voltage of 2000 V. By increasing the treatment duration, flow rate and applied voltage, the normalized % lysis could be increased. In addition, continuous and pulsed treatments yield similar normalized % cell lysis.
AB - We demonstrated a bacterial cell lysis method suitable for inhibitor resistant bacterial detection systems where purification of extracted DNA is not necessary. The presented method potentially improves the field portability of such systems. It allows cell lysis and DNA extraction to be performed without the use of bead mill, sonication, thermal cycling, additional reagents or enzymes. Bacterial cell lysis is achieved in a single step by pumping ozone generated by a micro corona discharge into the bacterial sample. The results with Pseudomonas putida as the target bacteria showed that it was capable of achieving 98.5 ± 0.2% lysis (normalized to 1 min of sonication at 10 W) after 10 min of treatment at a flow rate of 38 ml/min and an applied voltage of 2000 V. By increasing the treatment duration, flow rate and applied voltage, the normalized % lysis could be increased. In addition, continuous and pulsed treatments yield similar normalized % cell lysis.
KW - Bacterial detection systems
KW - Cell lysis
KW - Corona discharge
KW - Inhibitor resistant
KW - Ozone
UR - http://www.scopus.com/inward/record.url?scp=84928746240&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2015.04.030
DO - 10.1016/j.snb.2015.04.030
M3 - Article
AN - SCOPUS:84928746240
SN - 0925-4005
VL - 216
SP - 17
EP - 23
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -