TY - JOUR
T1 - Mhc multimer
T2 - A molecular toolbox for immunologists
AU - Chang, Jun
N1 - Funding Information:
This study was supported by a grant of Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of
Funding Information:
This study was supported by a grant of Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (grant No. HV20C0049 and HV20C0156).
Publisher Copyright:
© The Korean Society for Molecular and Cellular Biology. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The advent of the major histocompatibility complex (MHC) multimer technology has led to a breakthrough in the quantification and analysis of antigen-specific T cells. In particular, this technology has dramatically advanced the measurement and analysis of CD8 T cells and is being applied more widely. In addition, the scope of application of MHC multimer technology is gradually expanding to other T cells such as CD4 T cells, natural killer T cells, and mucosal-associated invariant T cells. MHC multimer technology acts by complementing the T-cell receptor-MHC/peptide complex affinity, which is relatively low compared to antigen-antibody affinity, through a multivalent interaction. The application of MHC multimer technology has expanded to include various functions such as quantification and analysis of antigen-specific T cells, cell sorting, depletion, stimulation to replace antigen-presenting cells, and single-cell classification through DNA barcodes. This review aims to provide the latest knowledge of MHC multimer technology, which is constantly evolving, broaden understanding of this technology, and promote its widespread use.
AB - The advent of the major histocompatibility complex (MHC) multimer technology has led to a breakthrough in the quantification and analysis of antigen-specific T cells. In particular, this technology has dramatically advanced the measurement and analysis of CD8 T cells and is being applied more widely. In addition, the scope of application of MHC multimer technology is gradually expanding to other T cells such as CD4 T cells, natural killer T cells, and mucosal-associated invariant T cells. MHC multimer technology acts by complementing the T-cell receptor-MHC/peptide complex affinity, which is relatively low compared to antigen-antibody affinity, through a multivalent interaction. The application of MHC multimer technology has expanded to include various functions such as quantification and analysis of antigen-specific T cells, cell sorting, depletion, stimulation to replace antigen-presenting cells, and single-cell classification through DNA barcodes. This review aims to provide the latest knowledge of MHC multimer technology, which is constantly evolving, broaden understanding of this technology, and promote its widespread use.
KW - Antigen-specific T cells
KW - MHC multimer
UR - http://www.scopus.com/inward/record.url?scp=85107085728&partnerID=8YFLogxK
U2 - 10.14348/molcells.2021.0052
DO - 10.14348/molcells.2021.0052
M3 - Short survey
C2 - 33972472
AN - SCOPUS:85107085728
SN - 1016-8478
VL - 44
SP - 328
EP - 334
JO - Molecules and Cells
JF - Molecules and Cells
IS - 5
ER -